‘阿里山’蝴蝶兰组织培养及其驯化移栽技术

Study on tissue culture and acclimatization technique in Phalaenopsis spp. cv. Ali Mountain

以蝴蝶兰新品种‘阿里山’为试材进行组培快繁研究,包括花梗腋芽不定芽的诱导、丛生芽增殖、生根培养以及组培苗炼苗移栽技术。结果表明,1/4MS+4 mg·L-1BA+0.3 mg·L-1NAA最适合不定芽诱导,诱导率可达82.5%;利用1/2MS+7 mg·L-1BA+0.8 mg·L-1NAA培养基进行培养时,丛生芽增殖系数达到3;利用改良1/2KC+1 mg·L-1NAA+100 g·L-1香蕉泥培养基培养时,生根率高达100%;组培苗驯化28 d,移栽成活率达93.3%。

The technology of tissue culture and rapid propagation of Pha/aetwps/s spp. cv. Ali Mountain was investigated in this ex- periment, including induction of axillary bud from pedicel, proliferation of bud clusters, rooting and transplanting technology. The results showed that 1/4MS ＋ 4 mg - L-1 BA ＋ 0.3 mg·L-1 NAA was best for induction of axillary bud, the induction rate was 82.5% ; 1/2MS ＋8 mg · L-1 BA ＋0.8 mg· L-l NKA was the optimum proliferaion medium, with the proliferaion coefficient of 3; 1/2KC ＋ 1 mg ·L-INAA ＋ 100 g · L-I banana mud was best for rooting, with the rooting rate as high as 100%. The transplanting survival rate was as high as 93.3% after acclimatization for 28 days.