抗炎或促炎细胞因子在新生儿坏死性小肠结肠炎发病过程中作用的临床分析

Clinical analysis on the role of anti-inflammatory or pro-inflammatory cytokines in the pathogenesis of neonatal necrotizing enterocolitis

目的:观察抗炎或促炎细胞因子在新生儿坏死性小肠结肠炎发病过程中的作用,为临床提供参考数据.方法:收集40例新生儿坏死性小肠结肠炎的血清,同时采集40例正常新生儿作为对照,使用酶联免疫吸附测定(ELISA)测定血清因子白介素IL-6、肿瘤坏死因子TNF-a和抗炎因子白介素IL-4的含量.结果:观察组新生儿抗炎因子IL-6、TNF-a水平明显高于对照组,差异均有统计学意义(P<0.01);观察组IL-4水平为(0.415±0.071)ng/L,对照组未见IL-4表达.结论:抗炎或促炎细胞因子在新生儿坏死性小肠结肠炎发病过程中明显升高,在治疗过程中应注意其水平变化.

AIM：To investigate the role of anti-inflammatory or pro-inflammatory cytokines in the pathogenesis of neonatal necro-tizing enterocolitis and thus provide reference data for clinical practice.METHODS：Necrotizing enterocolitis serum of 40 neo-natal cases （observation group）and normal serum of 40 healthy newborns （control group）were collected.The amount of IL-6, TNF-a and IL-4 was assessed by enzyme-linked immunosorbent assay （ELISA）.RESULTS：The amounts of IL-6 and TNF-a of observation group were significantly higher than those of control group.The difference was statistically significant （P 〈0.01 ）. The amount of IL-4 was （0.415 ±0.071）ng/L,while no IL-4 ex-pression has been observed in the control group.CONCLU-SION：The anti-inflammatory or pro-inflammatory cytokines in-creased significantly in the pathogenesis of neonatal necrotizing enterocolitis,so attention should be pained to its level during treatment.