摘要
目的:比较IHC与FISH检测乳腺浸润性导管癌HER-2的一致性。方法:分别用IHC和FISH对118例乳腺浸润性导管癌HER-2进行检测,比较两者检测结果的相关性。结果:118例浸润性乳腺导管癌标本,IHC检测结果为:14例为(-);20例为(1+),55例为(2+),29例为(3+)。FISH检测结果为:53例为>2.2,64例为<1.8,1例为1.8-2.2。IHC检测HER-2阳性的病例FISH检测中阳性符合率96.6%(28/29),IHC检测HER-2阴性的病例FISH检测均为阴性,符合率100%(34/34)。IHC检测HER-2(2+)的55例患者中有25例经FISH检测证实HER-2阳性,30例呈阴性。FISH检测共发现5例17号染色体多体,其发生率为4.24%。IHC检测到8例乳腺癌HER-2表达存在异质性,异质性在FISH检测中亦得到证实。结论:IHC与FISH检测乳腺浸润性导管癌HER-2具有高度一致性,免疫组化HER-2(2+)病例需行FISH检测。
Objective:To compare the consistency of HER-2 in breast infiltrating ductal carcinoma by IHC and FISH detection.Method:HER-2 of 118 cases with breast invasive ductal carcinoma were tested by FISH and IHC detection,and compared the results correlation.Result:118 specimens of invasive ductal carcinoma were seleced,The results tested by IHC were as follows:14 cases(-);20 cases(1+),55 cases(2+),29 cases(3+).The results tested by FISH were as follows:53 cases〉2.2,64 cases〈1.8,1 case was 1.8-2.2.HER-2 positive coincidence rate was 96.6%(28/29)by IHC detection in positive cases by FISH detection,HER-2 negative by FISH were in negative of IHC detection,the coincidence rate was 100%(34/34).25 HER-2 positive cases by FISH detection were among 55 cases of HER-2(2+)by IHC detection,and 30 were the negative.5 cases on chromosome multi-body in 17 were found by FISH detection,its incidence rate was 4.24%.HER-2 expression in 8 cases with of breast cancer exists heterogeneity by IHC detection,and the heterogeneity was also confirmed in FISH detected.Conclusion:HER-2 of breast infiltrating ductal carcinoma by IHC detection was high consistency with FISH detection,HER-2 cases(2+)by immunohistochemical need to be detected by FISH.
出处
《中国医学创新》
CAS
2015年第11期5-7,共3页
Medical Innovation of China
基金
梅州市科技计划项目(2012B68)