miR-199a在卵巢癌中表达下调及其对卵巢癌生物学功能的影响

The Down-Regulation of mi R-199a in Ovarian Cancer and Its Effect on Ovarian Cancer Biological Functions

目的:检测mi R-199a在上皮性卵巢癌组织中的表达水平以及在卵巢癌中参与的功能,初步探讨mi R-199a在卵巢癌发生、发展中的作用和机制。方法:通过实时定量逆转录聚合酶链反应(q RT-PCR)检测上皮性卵巢癌20例、良性上皮性卵巢肿瘤组织15例以及正常卵巢上皮细胞IOSE和1组具有不同侵袭能力的卵巢癌细胞株中mi R-199a的表达;构建mi R-199a的逆转录病毒质粒p MSCVpuro-mi R-199a并筛选稳定过表达mi R-199a的SKOV3细胞株;分别用CCK8和Transwell小室法检测过表达mi R-199a的SKOV3细胞在增殖、侵袭及迁移方面的改变。结果:mi R-199a在卵巢癌组的表达低于良性卵巢肿瘤组(P=0.001),mi R-199a在卵巢癌细胞中的表达低于正常卵巢上皮细胞IOSE(P<0.05),且在SKOV3中表达最低(P<0.01);与转染空载质粒SKOV3细胞相比,稳定转染p MSCVpuro-mi R-199a质粒的SKOV3细胞增殖、侵袭和迁移力均降低(P<0.000 1)。结论:mi R-199a在卵巢癌组织中表达降低,并在卵巢癌细胞增殖、侵袭及迁移过程中起抑癌基因的作用。

Objective： To study the expression and potential function of miR-199a in ovarian cancer occurrence and progression. Methods：Quantitative reverse transcriptase PCR（qRT-PCR） was used to detect the expression of miR-199a in 20 cases of epithelial ovarian cancers, 15 cases of benign epithelial ovarian tumors, ovary normal epithelial cell （IOSE） and ovarian cancer cells. Retrovirus expression vector pMSCVpuro-miR-199a was constructed and SKOV3 cells were transfected. CCK8 and transwell methods were used to detect the ability of proliferation and metastasis in SKOV3 cells after stably transfected with pMSCVpuro-miR-199a. Results：The mRNA expression of miR-199a in epithelial ovarian cancer group was significantly lower than that of in benign ovarian tumors （P=0.001）. Compared with IOSE cells,the miR-199a expression were down-regulated in ovarian cancer cells and expecially in SKOV3 cells （P 〈0.01）. The abilities of proliferation,migration and invasion in SKOV3 cells transfected with pMSCVpuro-miR-199a were significantly lower than that in pMSCVpuro-NC SKOV3 cells （P〈0.000 1 ）. Conclusions：Our data suggest that the expression of miR-199a was down-regulated in ovarian cancer and miR-199a might play as an anti-oncogene in ovarian cancer proliferation and metastasis.