不同产地白花蛇舌草强极性组分的亲水作用液相色谱-质谱联用分析

Analysis of strong hydrophilic components in Hedyotis diffusa Willd.from different sources by using hydrophilic interaction chromatography / electrospray mass spectrometry

目的利用亲水作用液相色谱-电喷雾离子源质谱联用(HILIC/ESI-MS)对白花蛇舌草药材中的强极性组分进行考察。方法在电喷雾离子源负离子模式下,采用基于基峰强度的特征色谱图谱中的8个共有峰为考察对象,对不同产地的白花蛇舌草及其伪品水线草中的强极性组分进行主成分分析。结果 8个共有峰的准分子离子或加合分子离子的质荷比分别为:m/z 383.2,m/z 399.2,m/z 389.1,m/z 850.9,m/z 665.3,m/z 827.4,m/z 989.4,m/z 1151.6。通过主成分分析,白花蛇舌草药材中的强极性组分具有明显的地域性,且其与伪品水线草成分差异也十分显著。结论 HILIC/ESI-MS方法可以提供强极性组分一些基本信息,较单纯利用色谱保留时间为参数的指纹图谱分析更加可靠、准确,为进一步了解白花蛇舌草强极性组分提供依据。

Objective To investigate the strong hydrophilic components in Hedyotis diffusa Willd. by using hydrophilic interaction chromatography/electrospray mass spectrometry （HILIC/ESI- MS）. Methods In ESI negative ion mode, 8 common peaks were detected under base peak intensity ehromatographie profile in the strong hydrophilic components from Hedyotis diffusa and its substitutes from different sources. The specific total ion chromatograms under base peak intensity were analyzed with principle component analysis method. Results The quasi - molecular ions or adduct molecular ions of the 8 peaks were m/z 383.2, m/z 399.2, m/z 389.1, m/z 850.9, m/z 665.3, m/z 827.4, m/z 989.4, m/z 1151.1, respectively. Based on the base peak intensity chromatographic profiles of the strong hydrophilie components from HILIC/ESI - MS, it can be seen that the strong hydrophilic components of Hedyotis diffusa exhibited distinct regional differences. Therefore, the strong hydrophilic components can be applied to distinguish Hedyotis diffusa from different places and its substitutes by using principle component analysis method. Conclusion - HILIC/ESI - MS method showed more information of the strong hydrophilic components, such as molecular information, which was helpful for further investigation of the strong hydrophilic components widely existed in traditional Chinese medicines.