疏肝理气法对功能性消化不良大鼠行为学及胃肠动力的影响

Effect of soothing the liver and regulating method on behavior and gastrointestinal motility in functional dyspepsia rats

目的探讨疏肝理气法对功能性消化不良大鼠行为学及胃肠动力的干预作用。方法 24只大鼠随机分为正常对照组、模型组、柴胡疏肝散组,每组8只。模型组、柴胡疏肝散组采用夹尾激惹刺激加不规则喂养法进行造模,柴胡疏肝散组组给予柴胡疏肝散水煎剂(1.41 kg/L)灌胃,其余两组给予等量生理盐水灌胃。每周进行敞箱实验(OPen-Field法)、糖水消耗实验的测定,4周后测定胃排空率及小肠推进率。结果 4周后,模型组大鼠出现扎堆、靠边,不喜活动,攻击性减少,易受惊吓,毛发干枯不顺等表现,柴胡疏肝散组上述表现相对不显著。4周后剖腹取胃,正常组胃粘膜无明显潮红、肿胀、糜烂等病变,模型组胃内残留物较多,部分胃粘膜潮红,柴胡疏肝散组上述表现不显著。模型组大鼠敞箱实验水平运动次数、糖水消耗、胃排空率及小肠推进率均较正常组显著减低(均P<0.05),柴胡疏肝散组大鼠糖水消耗、敞箱实验水平运动次数及胃排空率均较模型组增加(均P<0.05),柴胡疏肝散组敞箱实验垂直运动次数较模型组增多,但无显著性差别。结论疏肝理气法能有效改善功能性消化不良大鼠抑郁状态并能促进胃动力。

Objective To explore the effects of soothing the liver and regulating method on behavior and gastrointestinal motility of Functional Dyspepsia （FD） rats by a compound method of constantly squeezing their tails and hunger or overeating. Methods 24 rats were randomly divided into control group, model group , Chaihu Shugansan group （ n = 8 ）. The model group and Chaihu Shugansan group were established by by constantly squeezing rat tails and hunger or overeating. Chaihu Shugansan decoction （1.41 kg/L）was administered to Chaihu Shugansan group by gavage, other groups were administered saline. Open -field and sugar consumption test were detected weekly. After four weeks, gastric emptying and intestinal propulsion function were detected. Results After 4 weeks , rats in model group all showed pad batch, margination, move less, aggressive reduction, easy to rage , withered hair. The above manifestations in Chaihu Shugansan decoction were not so marked compared with model group. The rats were sacrificed and laparotomized after four weeks later, gastric mucosa of the control group had no significant flushing, swelling, erosion and other diseases, model group bad too much left over in stomach, flushing. The above manifestations in Chaihu Shugansan decoction were not so marked compared with model group. The sugar consumption, the scores in horizontal motion of Openfield test.gastric emptying and intestinal propulsion function were less in model group than control group（P 〈0.05 ）. The sugar consumption, the scores in horizontal motion of Open -field test, gastric emptying and intestinal propulsion function were more in Chaihu Shugansan group than model group （ P 〈 0.05 ）. The scores in vertical motion of Open - field test was more in Chaihu Shugansan group than model group, but no significant difference was observed. Conclusion The method of soothing the liver and regulating method not only alleviates depression, but also improves gastric motility.