补肾、健脾、活血方法对骨质疏松症小鼠骨及骨骼肌中Ca^(2+)-Mg^(2+)-ATP酶含量影响

Effect of Bushen Jianpi Huoxue Method to the Ca^(2+)-Mg^(2+)-ATP,enzyme content of bone and skeletal muscle in osteoporosis mice

目的:观察补肾、健脾、活血方法对骨质疏松症小鼠骨骼及骨骼肌中Ca2+-Mg2+-ATP酶含量的影响,探讨中医药防治骨质疏松症的作用机制。方法:实验药物灌胃8周后,ELISA法测定股骨及骨骼肌中Ca2+-Mg2+-ATP酶的含量。结果:补肾组、福善美组小鼠骨骼及骨骼肌的Ca2+-Mg2+-ATP酶较模型组明显升高。结论:骨质疏松症的形成可能与骨及骨骼肌Ca2+-Mg2+-ATP酶含量异常有关;补肾、健脾方法通过调控骨质疏松症小鼠的骨及骨骼肌Ca2+-Mg2+-ATP酶含量,起到防治骨质疏松症的作用。

Objective： To observe the effects of tonifying kidney,tonifying spleen, promoting blood flow therapies on Ca^2＋ -Mg^2＋-ATP enzyme content of bone and skeletal muscle in osteoporosis mice, so as to investigate the mechanism of traditional Chinese medicine in prevention and treatment of osteoporosis. Methods： Intragastric administration was the way of medication for 8 weeks , and then the bone and skeletal muscle were taken to detemermine the content of Ca^2＋ -Mg^2＋- ATP enzyme by ELISA method. Results： the bone and the skeletal muscle Ca^2＋ -Mg^2＋-ATP enzyme content increased significantly in the tonifying kidney medicine group and the Fosamax group. Conclusion： The formation of osteoporosis may be related to abnormal content of bone and skeletal muscle Ca^2＋ -Mg^2＋-ATP enzyme. The tonifying kidney method , tonifying spleen method are of a preventive effect on osteoporosis through the regulation of content of osteoporosis mice bone and skeletal muscle Ca^2＋ -Mg^2＋-ATP enzyme.