中华按蚊GSTO1基因的鉴定及生物信息学分析

Identification and Bioinformatics Analysis of the GSTO1 Gene in Anopheles sinensis

谷胱甘肽S-转移酶(GST)是有机体内一类具有解毒和抗氧化等多功能的超家族蛋白酶。在昆虫中GST主要分为七大家族,Omega家族(GSTO)为研究较少的家族。在本研究中,通过Blast方法搜索中华按蚊(Anopheles sinensis)转录组数据,得到中华按蚊GSTO1cDNA序列,全长1 059bp,其中阅读框ORF长744bp,编码248个氨基酸。推测该基因编码蛋白的分子量和等电点分别为28.5kD和6.92。分析发现该蛋白不存在跨膜区和信号肽,亚细胞定位预测显示该蛋白质位于细胞质中。同源性分析表明GSTO1蛋白与达氏按蚊(Anopheles darlingi)氨基酸序列相似性最高。本研究进一步丰富了GST蛋白的基础数据,为进一步研究昆虫GSTO蛋白的功能奠定了理论基础。

Glutathione S-transferees （GSTs） are superfamily of multifunctional enzymes, some of which have been reported to be rel- ative to insecticide resistance. In insects, GSTs have been classified into seven families, including Delta, Eplison, Omega, Sigma, Theta, Zeta and Unclassified. The study identified genes in the family Omega, named as AsGSTO1, from transcriptome in Anopheles sinensis. The full-length cDNA of the gene was 1059 bp with an opening reading frame （ORF） of 744 bp encoding 247 amino acids. The calculated molecular weight of AsGST01 is 28.5 kDa and the estimated pI is 6.92. The characteristics, homology and phylogenetic relationships of the AsGSTO1 were carried out through bioinformatic analyses. The AsGSTO1 shares the highest sequence similarity （92%） with the homolog of Anopheles darling. This work provides a base information frame for further research of the AsGST01 gene.