摘要
目的 了解2011-2014年青岛地区人群甲型H3N2流感病毒流行株基因进化及抗原变异趋势.方法 选取2011-2014年间青岛地区流行的甲型H3N2流感病毒64株,提取病毒RNA,应用逆转录聚合酶链反应（RT-PCR）扩增HA、NA、MP 3个基因片段,并进行序列测定,对各基因片段进行系统发育分析及基因和氨基酸位点变异分析.结果 HA进化树分析表明,甲型H3N2流感病毒基本上分为三大分支,并且每个分支与当年的疫苗株都不在同一分支上;HA1蛋白抗原决定簇共有8个位点发生了变化;NA蛋白酶活性中心及周围相关位点氨基酸组成保守,未检测到耐奥司他韦和扎那米韦的变异位点.M2蛋白均发生S31N突变.结论 2011-2014年青岛地区流行的H3N2流感病毒在持续不断地发生基因变异而产生抗原漂移;毒株全部为烷胺类药物耐药株,但对神经氨酸酶抑制剂敏感.
Objective To study the prevalence and variation of influenza A （H3N2） viruses circulating in Qingdao from 2011 to 2014.Methods RNA of 64 influenza A/H3N2 virus isolates circulated in Qingdao from 2011 to 2014 was extracted.HA,NA and MP segments were amplified and sequenced.Each sequence data was analyzed using Sequencher,phylogenetic analysis using the MEGA5.0.Results HA1 gene phylogenetic tree analysis showed that there was three branches in 2011-2012,2012-2013,2013-2014 respectively,and each branch had nohomology with the vaccine strains of the same year.Compared with the vaccine strains,eight antigen sites were substituted in HA1 area;Residues at the enzyme active sites of NA protein were strictly conservative,and no change was observed in amino acid residues which were related to drug resistance against osehamivir and zanamivir.All the isolations had a S31N mutation in M2 protein.Conclusions All the A H3N2 influenza virus circulated in Qingdao from 2011 to 2014 presented continual variation and therefore caused antigenic drift.All the isolations were adamantane-resistance,but susceptible to inhibitors of neruaminidase.
出处
《国际病毒学杂志》
2015年第2期99-105,共7页
International Journal of Virology
基金
青岛市公共领域科技支撑计划项目（10-3-3-8-1-nsh）
