病毒感染与COPD的关系及在COPD发病机制中的作用分析

Analysis of the relationship between virus infection and COPD and its role in the pathogenesis of COPD

目的 观察临床慢性阻塞性肺病（COPD）病毒感染状况,探讨其与COPD的关系以及在COPD发病机制中的作用.方法 选取120例自2012年9月至2013年12月来我院治疗的COPD患者,将临床检测83例COPD急性加重期患者归为A组,37例COPD稳定期患者归为B组,21名相关病毒感染检测IgM阳性患者为D组,选取19例正常人对照作为C组.采用生物薄片技术,检测出三组（A、B、C）的血清中呼吸道合胞病毒（RSV）、腺病毒（ADV）、巨细胞病毒（CMV）、柯萨奇病毒（COX）的特异性抗体IgM、IgG,以流式细胞术检测D组和C组T淋巴细胞亚群CD3、CD4、CD8.结果 A组IgM阳性率与B组、C组有极明显差异（P＜0.001）;A组IgG阳性检出率与B组无明显差异（P＞0.05）,C组与前两组差异明显（P＜0.01）.D组与C组T细胞亚群的CD4、CD8及CD4/CD8的检测结果有差异（P＜0.05）,CD3的检测结果无明显差异（P＞0.05）.结论 病毒感染通过影响机体免疫系统,导致免疫系统功能失调从而诱发COPD发生或者加重,病毒感染与COPD的发生有相关性.

Objective To observe the infection status of clinical COPD virus,and explore its relationship with COPD and the role in the pathogenesis of COPD.Methods 83 patients with acute exacerbation of COPD were selected as group A,37 cases of COPD patients in stable phase were collected as group B,19 cases of normal control were selected as group C,21 cases of specific IgM antibody positive patients were collected as group D From 2012 September to 2013 December.Specific antibodies IgM,IgG in the serum of respiratory syncytial virus （RSV）,adenovirus （ADV）,cytomegalovirus （CMV）,Coxsackie virus （COX） using the biochip technology.CD4,CD8 group,T lymphocyte subsets of group D and C were detected by flow cytometry.Results The IgM positive rate of group A has a very significant difference compared with group B or group C （P 〈0.001）.The IgG positive rate of group A and B have no significant difference （P 〉 0.05）.There was significant difference of CD4 、CD8 and CD4/CD8 between group C andgroup D （P 〈 0.05）.Conclusions Immune system was affected with viral infection,which lead to induce COPD or aggravate.