RANTES基因多态性与结核性脓胸发病风险的关系

The correlation between RANTES gene polymorphism and the onset risk of tuberculous empyema

目的：探讨河北地区受激活调节正常 T 细胞表达和分泌因子（regulated upon activation normal T cell ex－pressed and secreted factor，RANTES）基因－403G／A 和－28C／G 单核苷酸多态性（SNP）与结核性脓胸的关系。方法采用聚合酶链反应－限制性片段长度多态性（PCR－RFLP）方法检测300例结核性脓胸患者和300例对照人群RANTES基因－403G／A 和－28C／G 的基因型。结果 RANTES 基因－403G／A SNP 基因型频率和等位基因频率在结核性脓胸组和正常组间差异有统计学意义（ P ＝0．004和 P ＝0．008），与 GG 基因型相比，携带 AG ＋AA 基因型可增加结核性脓胸发病风险（OR ＝1．40395％，CI ＝1．015～1．939）。 RANTES 基因－28C／G SNP 基因型频率和等位基因频率在结核性脓胸组和正常组间差异无统计学意义（ P ＝0．128和 P ＝0．064），与 CC 基因型相比，携带 CG ＋GG 基因型与结核性脓胸发病风险无关（OR ＝1．31495％，CI ＝0．891～1．936）。结论 RANTES 基因－403G／A SNP 可能与结核性脓胸的发病风险有关，即携带 AG ＋AA 基因型增加结核性脓胸发病风险；RANTES 基因－28C／G SNP 可能与结核性脓胸发病风险无关。

Objective To investigate the correlation between RANTES-403G/A and-28C/G single nucleotide polymorphism （SNPs） and the onset risk of tuberculous empyema （TE） in Hebei Province,China.Methods The gene types of SNPs of RANTES-403G/A and-28C/G were detected by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） in 300 patients with tuberculous empyema （patient group） and 300 healthy subjects （control group）.Results There were significant differences in genotype frequency and allele frequency of RANTES-403G /A SNP between the two groups （P =0.004,0.008,respectively）.As compared with GG genotype,the AG ＋GG genotype could increase the onset risk of tuberculous empyema （OR =1.403 95%,CI =1.015 ~1.939）.However there were no significant differences in genotype frequency and allele frequency of RANTES-28C/G between the patient group and control group （ P =0.128, 0.064, respectively）.As compared with the CC genotype,the CG ＋GG genotype was not correlated to onset risk of TE （OR =1.314 95%,CI =0.891 ~1.936）.Conclusion The RANTES-403G/A SNP may be correlated to onset risk of TE,carrying G allele （AG ＋GG） may increase onset risk of TE.However 28C/G SNP may be not related with onset risk of TE.