响应面优化超声辅助提取无机砷

Optimization of Ultrasonic-assisted Extraction of Inorganic Arsenic

在单因素试验基础上,采用三因素三水平的Box-Behnken试验确定林下参茎中的无机砷提取条件。在实验范围内各因素对无机砷的提取影响程度大小依次为:提取时间>HCl浓度>提取温度。最优工艺参数为HCl浓度7.24mol/L,提取温度为43.7℃,提取时间为36min。理论含量为86.82ng/g,实际测得88.18ng/g,相对误差为1.56%。

A three-variable and three-level Box-Behnken experimental design was used to optimize the extraction process conditions of inorganic arsenic in the stem of wild ginseng based on the results of one-factor-at-a-time method.The effect of the three process conditions on the content of inorganic arsenic decreased in the following order：extraction time 〉HCl concentration 〉extraction temperature.The optimum extraction conditions were：the extraction concentration of 7.24 mol/L,extraction temperature at 43.7℃ and extraction time of 36 min.The content of inorganic arsenic in theory and actual extraction process were 88.18ng/g respectively and 86.82ng/g with a relative error of 1.56%.