摘要
目的研究金雀异黄素对SMMC-7721细胞系肝癌干细胞样细胞特性以及氟尿嘧啶敏感性的影响。方法不同浓度金雀异黄素处理SMMC-7721细胞系球形成细胞。肿瘤球形成法检测自我更新能力。MTT比色法测定氟尿嘧啶药物敏感性。Western blot分析干细胞标志物CD133、CD44、Gli1和ABCG2蛋白表达。结果金雀异黄素显著抑制SMMC-7721细胞肿瘤球形成,并优先抑制球形成细胞增殖活性(P<0.05)。金雀异黄素(10μmol/L)有效逆转球形成细胞对氟尿嘧啶的耐药性。金雀异黄素降低CD133、CD44、Gli1和ABCG2蛋白表达水平。结论金雀异黄素抑制肝癌干细胞样细胞特性和氟尿嘧啶耐药性,其作用机制与抑制干细胞标志物蛋白表达和阻断Hedgehog信号传导有关。
Objective To investigate the effects of genistein on the characteristics of liver cancer stem-like ceils （LCSLCs） derived from SMMC-7721 cell line and the sensitivity to fluorouracil. Methods Sphere-forming cells （SFCs） from SMMC-7721 cell line were treated with various concentrations of genistein. The capability of self-renewal was tested using tumor sphere-forming assay. The sensitivity to fluorouracil was determined by MTT assay. And the protein expression of stem cell markers CD133, CD44, Hedgehog （Hh） signal effector Glil and its target molecule ABCG2 was analyzed using Western blot. Results Genistein significantly inhibited the capability of sphere-forming in SMMC-7721 SFCs and relative selectively suppressed the proliferation activity of SFCs （P〈0.05）. Genistein （10 g mol/L） could effectively reverse the resistance of SFCs to fluorouracil. And genistein could also down-regulate the protein expression of CD133, CD44, Glil and ABCG2. Conclusion Genistein inhibits characteristics of liver cancer stem cells and resistance to Fluorouracil, and its mechanism is likely involved in inhibiting protein expression of stem cell markers and obstructing Hh signal pathway.
出处
《海南医学》
CAS
2015年第8期1108-1111,共4页
Hainan Medical Journal
