摘要
目的探讨血清微小RNA(miR)-520g在子痫前期(PE)患者孕期的变化及意义。方法分别提取30例PE患者和30名正常妊娠者早、中、晚孕期血清中的循环miR,利用实时荧光定量逆转录聚合酶链反应(PCR)检测血清中的miR-520g的表达量,比较两组间不同孕期miR-520g的差异。结果正常妊娠组随孕程进展血清miR-520g的表达量增加;与正常妊娠组相比,PE组孕早期miR-520g显著增加,PE组孕中、晚期的血清miR-520g表达量与正常妊娠组差异无统计学意义。结论孕早期血清miR-520g可能是PE预测的良好标志物。
Objective To investigate the change and significance of serum microRNA （ miR）-520g in preeclampsia among pregnant patients .Methods Serum cycling miR was extracted from 30 patients with preeclampsia and 30 healthy pregnant women at early , middle and late pregnant stages .The expression of miR-520g was determined by quantitive reverse transcription polymerase chain reaction （ PCR ） , and the difference between the 2 groups was analyzed statistically.Results MiR-520g increased with pregnant days in healthy pregnant group .MiR-520g in early pregnant stage of preeclampsia group increased comparing with that of healthy pregnant group , whereas no significant difference was observed in middle and late pregnant stages between preeclampsia group and healthy pregnant group .Conclusions Serum miR-520g in early pregnant stage may be a potential biomarker for the prediction of preeclampsia .
出处
《检验医学》
CAS
2015年第4期345-348,共4页
Laboratory Medicine
基金
上海市卫生局青年科研项目(20124Y080)
