2型糖尿病肾病患者外周血 IL-17和 IL-22 mRNA 水平

Levels of IL-17 and IL-22 mRNA in the Blood of Type 2 Diabetic Nephropathy Patients

目的：研究2型糖尿病（T2DM）患者肾脏不同病变时期外周血 IL-17和 IL-22 mRNA 水平，探索糖尿病肾病（DN）患者 IL-17和 IL-22基因表达水平与肾脏病变的关系。方法实验包括60名2型 T2DM 患者和20名正常对照者（NC），其中糖尿病患者包括：无蛋白尿组（NA，n＝22）、微量清蛋白尿组（MA，n＝18）和糖尿病肾病组（DN，n＝20）。采用实时荧光定量 PCR 方法检测 IL-17和 IL-22 mRNA 水平，分析各组外周血 IL-17和 IL-22 mRNA 水平差异。结果DN 组 IL-17和 IL-22 mRNA 水平显著高于 MA 组，NA 组和 NC 组差异有统计学意义（P ＜0.01）；MA，NA 和 NC 三组之间 IL-17和IL-22 mRNA 差异无统计学意义（P ＞0.05）。结论在2型糖尿病肾病外周血中 IL-17和 IL-22 mRNA 水平升高，IL-17和 IL-22可能在 DN 病变中发挥作用。

Objective To investigate levels of IL-17 and IL-22 mRNA in the blood of type 2 diabetes（T2DM）patients with the different stages of kidney injury and explore the relationship between the gene expression levels of IL-17,IL-22 and renal lesions in patients with diabetic nephropathy（DM）.Methods Subjects included 60 T2DM patients with or without kidney injury and 20 normal controls（NC,n=20）.Diabetes patients were divided into 3 groups by level of urinary albumin to creatinine ratio （ACR）：no proteinuria group （NA,ACR〈30 mg/g,n=22）,microalb uminuria group （MA,30 mg/g〉ACR〉300 mg/g,n=18）and diabetic nephropathy group （DN,ACR〉300 mg/g,n=20）.Quantitative Real-Time RT-PCR was used to detect IL-17 and IL-22 mRNA levels.Analysis differences of IL-17 and IL-22 mRNA levels among NA,MA,DN and NC groups.Results The levels of IL-17 and IL-22 mRNA were significantly higher in DN group than that in MA,NA and NC group （P 〈0.01,respectively）,However,there were not significant difference among MA,NA and NC group （P 〉0.05,respectively）.Conclusion Levels IL-17 and IL-22 mRNA were increased in blood of T2DM patients with nephropathy.IL-17 and IL-22 may play role in the pathogenicity of diabetic nephropathy.