子痫前期患者血清中 TFAR19活性研究

Serum TFAR19 Activity in Preeclampsia Patients

目的：观察子痫前期患者血清 TFAR19活性。方法选取北京市怀柔区妇幼保健院住院初产行剖宫产术的子痫前期患者43例，其中重度子痫前期患者18例、轻度子痫前期患者25例，同时随机选取无妊娠并发症的初产剖宫产产妇40例设为对照组，于入院次日采集外周静脉血，采用酶联免疫吸附试验（enzyme-linked immunosorbent assay，ELISA）检测子痫前期患者血清中 TFAR19活性，即 TFAR19催化产生的吸光度 A。结果重度子痫前期、轻度子痫前期、正常对照组TFAR19活性分别为43.56±2.93，33.38±2.32和23.63±2.94活性单位，各组之间 TFAR19活性差异具有统计学意义（F ＝10.65，P ＜0.01）。与正常对照组相比，重度子痫前期组（q ＝6.384，P ＜0.001）、轻度子痫前期组（q ＝3.478，P ＝0.016）TFAR19活性差异均具有统计学意义。重度子痫前期组、轻度子痫前期组之间 TFAR19活性差异有统计学意义（q＝2.993，P ＝0.037）。各组之间年龄差异无统计学意义（F ＝0.091，P ＝0.913）。各组之间孕周、新生儿体重差异有统计学意义（F ＝4.37，P ＝0.016；F ＝35.06，P ＝0.00）。结论TFAR19的增加可促进机体细胞凋亡，参与子痫前期的发生，并且 TFAR19的含量可随病情的严重程度而增加。

Objective To explore serum TFAR19 activity in preeclampsia patients.Methods 43 of cesarean delivery patients with preeclampsia in the Huairou Maternal and Child Health Hospital of Beijing were recruited and the peripheral venous blood was collected.Among them,severe preeclampsia patients were 18,mild preeclampsia were patients 25.In addition,40 unrelated healthy cesarean puerpera from a routine health survey were enrolled as controls.Enzyme-linked immunosorbent assay（ELISA）was used to detect serum TFAR19 activity in preeclampsia patients.That was absorbancy-A catalytic generated by TFAR19.Results The activity of TFAR19 severe preeclampsia patients,mild preeclampsia patients,and healthy controls were 43.56±2.93,33.38±2.32 and 23.63±2.94,respectively.The TFAR19 activity in each group was statistically significant difference （F = 10.65,P 〈0.01）.Compared with healthy controls,the TFAR19 activity in severe preeclampsia group （q=6.384,P 〈0.001）,mild preeclampsia group （q = 3.478,P =0.016）was statistically significant difference.The TFAR19 activity in preeclampsia patients subgroups （severe preeclampsia group,mild preeclampsia group）was statistically significant difference （q = 2.993,P = 0.037）.Age between each groups was no-statistically significant difference （F =0.091,P =0.913）.Gestation age and neonatal weight betweeneach groups was statistically significant difference （F=4.37, P =0.016;F=35.06,P 〈0.001）.Conclusion The increase of TFAR19 could promote apoptosis,participating the occurrence of preeclampsia.The content of TFAR19 could be increased with the severity of the preeclampsia.