两种试剂检测抗心磷脂抗体的比较

Comparison of Two Kits in Determining Anti-Cardiolipin

目的：对两种商品化的抗心磷脂抗体（Anti-cardiolipin，ACA）试剂的检测结果进行比较。方法采用欧蒙公司ACA-IgA／G／M（总 ACA）检测试剂和亚辉龙公司 ACA-IgG，IgM 和抗β2糖蛋白 I（β2GPI）抗体 IgG（ACA 3项）测定试剂盒，同时检测66例血清样本，比较两者结果的差异，并分析两者之间的关系。结果欧蒙总 ACA 试剂的检出阳性率为37.88％（25／66）；亚辉龙 ACA 3项试剂的检出阳性率为31.82％（21／66，ACA-IgG，IgM 和抗β2GPI IgG 三者任一阳性），两种试剂检测结果不具有统计学差异；两者的检测符合率为87.88％（58／66）。欧蒙试剂 ACA-IgA／G／M 的检测结果与亚辉龙试剂 ACA-IgG，IgM 和抗β2GPI IgG 3个检测值的总和具有良好的相关性（r 2＝0.892，P ＜0.01）。结论两种试剂检测结果符合性较高，具有良好的相关性，能满足临床应用的需要；两种试剂各具特色，可以根据需要选择合适的试剂单独或联合进行检测。

Objective To compare the results of anti-cardiolipin （ACA）measuring by two commercial available kits.Methods ACA in total of 66 serum samples were both determined by kits from Euroimmun and YHLO simultaneously,then the results were analyzed comparatively and correlatively.The Euroimmun kit was applied to determination the level of ACA-IgA/G/M,and the YHLO kit determined ACA-IgG/M and anti-β2-glycoprotein I antibody （β2GPI IgG）.Results The positive rate by Euroimmun kit was 37.88% （25/66）,while 31.82% （21/66）was positive （one positive among ACA-IgG,IgM andβ2GPI IgG）when determined by YHLO kit,and there was no significant difference between the two kits.The accordance rate of the two kits was 87.88% （58/66）.The ACA-IgA/G/M value by Euroimmun kit and the summation of ACA-IgG, IgM andβ2GPI IgG by YHLO kit showed well linear correlation （r^ 2 =0.892,P 〈0.01）.Conclusion Results from the two kits were consistent and correlated well,and they are suitable for the clinical application;these two kits have their own char-acteristics,which could be used by individual or combination accordingly.