摘要
为了优化猪的手工克隆技术方案,从而提高猪手工克隆效率,通过比较不同浓度(2和10 ng/m L)的猪粒细胞-巨噬细胞集落刺激因子(PGM-CSF)对孤雌胚胎和克隆胚胎的囊胚发育率及囊胚细胞数的影响,并将PGM-CSF处理过的手工克隆囊胚进行移植,比较对照组和试验组的妊娠率、产仔率及克隆整体效率。结果显示:用10 ng/m L PGM-CSF处理重构胚后,试验组胚胎细胞数统计略高于对照组((47.74±3.88)vs(43.69±3.72)),囊胚率高于对照组((39.10±0.01)%vs(36.27±0.02)%),但差异均不显著(P>0.05);试验组10只受体中有5只怀孕且分娩,而对照组10只受体只有1只怀孕且分娩;试验组出生仔猪存活率略高于对照组(36.36%vs 30.00%),克隆总效率略高于对照组(0.37%vs 0.30%),但没有显著差异(P>0.05)。综上所述,PGM-CSF有利于提高猪手工克隆胚胎整体发育能力,有提高猪手工克隆对受体的利用率的潜在可能性。
The present study was conducted to improve the efficiency of porcine hand-made clone from optimizing technology solutions.Firstly,we compared effects of different concentrations of PGM- CSF（ 2 ng / m L,10 ng / m L） on in vitro development of porcine parthenogenetic embryo and SCNT embryos,including blastocyst formation rate and cell number per blastocyst,and then we transferred embryos into surrogates to compare the pregnancy rate,farrowing rate,and overall efficiency of control group and treatment group. The results showed that a higher cell number per blastocyst（ 47. 74 vs 43. 69）,blastocyst formation rate（ 39. 10% vs 36. 27%）,pregnancy rate（ 50. 00% vs10. 00%）,farrowing rate（ 36. 36% vs 30. 00%）,and overall efficiency（ 0. 37% vs 0. 30%） were found in the treatment group but were no significant differences. In conclusion,PGM- CSF is helpful to the overall development of porcine embryos and improve potentially the efficiency of porcine hand- made clones.
出处
《畜牧与兽医》
北大核心
2015年第4期20-24,共5页
Animal Husbandry & Veterinary Medicine
基金
深圳市战略性新兴产业发展专项资金(JCYJ20120830105157538)
深圳市生物
互联网
新能源
新材料产业发展专项资金(CYC201105250009A)
深圳市海外高层次人才创新创业专项资金(KQC201109050086A)
