摘要
目的:探讨辣椒素受体在神经压榨性损伤后再生过程中的作用。方法:采用大鼠坐骨神经压榨性损伤在体模型,分别记数损伤前给予或不给予辣椒素受体拮抗剂的情况下,损伤发生1、2周后的健侧、损伤近端、损伤中端及损伤远端横断面的髓神经纤维数目并进行统计学分析。结果:(1)健侧、损伤近端各组别间无统计学差异(P>0.05);(2)坐骨神经损伤中段的神经轴突数量计数发现:单纯损伤1周与损伤2周相比、AMG517处理+损伤后1周与2周相比、单纯损伤2周与AMG517+损伤2周之间相比,其轴突数量的差异均有统计学意义(P<0.01);采用AMG517处理组大鼠坐骨神经损伤部位的轴突数量明显多于单纯损伤组。(3)在坐骨神经损伤远端,其神经轴突的数量随着损伤时间的延长轴突数量有所增加,尤其是损伤前采用AMG517处理组大鼠损伤远端神经轴突的数量明显增多,差异有统计学意义(P<0.01)。结论:阻断TRPV1功能可促进大鼠坐骨神经压榨性损伤后的修复。
Objective： To observe the effect of the transient receptor potential vanilloid 1 （ TRPV1 ） on neuronal regeneration after crush injury to the rat＇s sciatic nerve.Method： The right sciatic nerve of the adult male Sprague-Dawley rats were crushed.Rats in the experimental group received a subcutaneous injection of AMG-517 , a highly potent and selective TRPV1 antagonist, into the right hind paw 1 h before the crush.At the crush zone, proximal zone and distal zone, the numbers of myelinated fibers on the transverse section were counted after a surviving time of 7 or 14 days, Result： （ 1 ） At the intact side and the proximal zone, the numbers of myelinated fibers did not show any difference. （ 2 ） the sciatic nerve injury in the middle of neural axon number counting found that damage to 1 week compared with in 2 weeks, AMG517 treatment＋l week after injury compared with 2 weeks, pure injury and compared between AMG517 ＋ damage, its axon number difference had statistical significance （ P〈0.01 ） .Use AMG517 treatment group rats sciatic nerve injury of axon number significantly more than the simple injury group. （ 3 ） In the distal sciatic nerve injury~ the nerve axon number of damage extended axon number increased with time, especially the damage before used AMGS17 treatment group rats damage distal nerve axon number increased obviously, the difference was statistically significant （ P〈0.01 ） , Conclusion： Blockage of the TRPV 1 can promote neuronal regeneration after sciatic crush injury,
出处
《中国医学创新》
CAS
2015年第12期26-30,共5页
Medical Innovation of China
基金
国家自然科学基金面上项目(81171178)
山西省自然科学基金项目(2012011036-3)
山西省归国人员科研资助项目(2013011054-2)
