期刊文献+

Electroacupuncture down-regulates the expressions of colonic NGF and NGFR in visceral hypersensitivity rats 被引量:5

电针下调内脏高敏感性大鼠结肠NGF和NGFR的表达(英文)
原文传递
导出
摘要 Objective:To observe the effect of electroacupuncture (EA) on the expressions of colonic nerve growth factor (NGF) and nerve growth factor receptor (NGFR) in visceral hypersensitivity rats, and investigate the mechanisms of EA on chronic visceral hypersensitivity. Methods: A total of 24 neonatal rats were randomly divided into a normal group, a model group and an EA group. The models of visceral hypersensitivity rats were prepared according to the method of Al-Chaer. The rats in the EA group were treated with EA at Tianshu (ST 25) and Shangjuxu (ST 37), 20 min for each time, once a day, for 7 consecutive days. After the first treatment, the observation of abdominal withdrawal reflex (AWR) was used to evaluate the pain threshold of visceral hypersensitivity rats. After 7-day treatments, the colonic tissue of rat was collected to test the immunohistochemistry of NGF and NGFR. Results: The score of AWR in the model group was higher than that in the normal group, but the score decreased after EA treatment. The positive expressions of colonic NGF and NGFR in the model group were increased than those in the normal group (P〈0.05), while they were both decreased in the EA group after treatment (P〈0.001). Conclusion: The EA could up-regulate the pain threshold of visceral hypersensitivity rats, and down-regulate the expressions of colonic NGF and NGFR. The regulation of the expressions of colonic NGF and NGFR would be one of the peripheral mechanisms of EA in treating chronic visceral hypersensitivity. 目的:观察电针对内脏高敏感大鼠结肠神经生长因子(nerve growth factor,NGF)和神经生长因子受体(nerve growth factor receptor,NGFR)表达的影响,探讨电针治疗慢性内脏高敏感性的作用机制。方法:将24只新生乳鼠随机分为正常组、模型组和电针组。参照A l-Chaer法制备内脏高敏感性大鼠模型。电针组予电针天枢、上巨虚治疗,每次20分钟,每天1次,连续7天。首次治疗后,通过观察大鼠腹壁撤回反射(abdominal withdrawal reflex,AWR)评分以评价内脏高敏感性大鼠的痛阈。治疗7天后,采集大鼠结肠组织进行N GF和N GFR免疫组织化学检测。结果:模型组大鼠的A WR评分均高于正常组,经电针治疗后均降低。模型组大鼠的结肠N GF和N GFR阳性表达较正常组显著增加(P<0.05),电针组治疗后N GF和N GFR阳性表达均显著降低(P<0.001)。结论:电针可使内脏高敏感性大鼠的痛阈升高,并降低结肠的N GF和N GFR表达。电针对结肠N GF和N GFR表达的调节可能是其治疗慢性内脏高敏感性的外周作用机制之一。
出处 《Journal of Acupuncture and Tuina Science》 CSCD 2015年第2期67-73,共7页 针灸推拿医学(英文版)
基金 supported by Project of Shanghai Natural Sciences Foundation,No.14ZR1438700 National Basic Research Program of China (973 Program),No.2015CB554500 The Second Period Xinglin Scholars Program of Shanghai University of Traditional Chinese Medicine National Natural Sciences Foundation of China,No.81202752~~
关键词 Acupuncture Therapy ELECTROACUPUNCTURE V isceral Pain Irritable Bowel Syndrome Nerve Growth Factor Receptors Nerve Growth Factor HYPERALGESIA 针刺疗法 电针 内脏痛 肠易激综合征 神经生长因子 受体,神经生长因子 痛觉过敏
  • 相关文献

参考文献6

二级参考文献68

共引文献66

同被引文献76

引证文献5

二级引证文献18

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部