右归饮促进骨折愈合的最佳浓度的实验研究

Experimental Study of Fracture Healing Promotion with the Best Concentration of Youguiyin

目的:观察右归饮不同浓度促进骨折愈合的效果,获得右归饮治疗骨折的最佳浓度,并获得右归饮治疗的最佳时间。方法:将120只SD雄性大鼠,体质量(250±20)g,随机分为4组:骨折组、低浓度右归饮+骨折组、中浓度右归饮+骨折组、高浓度右归饮+骨折组,每组6只。造模完成后,骨折组用生理盐水灌胃,右归饮组灌服不同浓度右归饮汤剂,于造模后3、7、14、21、28 d处死大鼠取骨痂进行影像形态学及QPCR检测。结果:在各个时间点高、中、低浓度右归饮组均较对照组骨痂量明显增多,VEGF表达量显著升高(P<0.01),表达高峰出现时间明显提前、维持时间延长,且与浓度呈一定的正相关。结论:右归饮能明显促进骨折愈合,高浓度为治疗骨折的最佳浓度,并且应在早期即开始使用。

Objective ： To observe the effect of Youguiyin in different concentrations in promoting healing fracture, get the best concentration of Youguiyin in the treatment of fracture, and get the best time of treatment. Methods ：120 male SD rats, weight （250 ＋ 20g）, were randomly divided into 4 groups：fracture group, small concentration of Youguiyin ＋ frac- ture group, middle concentration of Youguiyin ＋ fracture group, large concentration of Youguiyin ＋ fracture group, 6 rats in each group, fracture group with physiological saline, Youguiyin group were administrated with different concentrations of Youguiyin,3D, 7 d, 14 d, 21 d, 28 d after modeling, the rats were sacrificed to get the bone callus image morphology and QPCR detection. Results ： in each time point of large, middle and small concentration of Youguiyin group , the callus were more than those in the control group, the expression level of VEGF increased significantly （P 〈 0.01 ）, the expres- sion of peak time significantly ahead of schedule, the maintenance time is lengthened, and had a positive correlation with the concentration of. Conclusion：Yougui Yin can significantly promote the healing of fracture, high concentration is the optimum concentration for the treatment of fracture, and shall begin to use in the early.