液相色谱质谱联用法测定卷柏中熊果苷的含量

Determination of arbutin in Selaginella tamariscina( Beauv. ) Spring by HPLC- MS / MS method

目的：采用高效液相色谱质谱联用（LC-MS/MS）方法测定卷柏药材中的熊果苷的含量。方法：色谱柱为Diamonsil C18（5μm,150 mm×4.6 mm,i.d.）色谱柱,流动相为为乙腈-0.4%甲酸（80∶20,v/v）,流速为0.6 m L·min-1,柱温为室温,进样量为10μL;采用电喷雾离子源进行正离子模式检测,选择性反应检测模式（SRM）用于定量测定。结果：熊果苷在1.0~150μg·m L-1范围内与峰面积线性关系良好,低、中、高3个水平的加样回收率分别为98.8%、98.8%和99.7%,RSD均小于1.1%。结论：该方法简便,准确,重现性好,可用于卷柏中熊果苷的含量测定和质量控制。

Objective ： To determine the contents of arbutin in Selaginella tamariscina（ Beauv. ） Spring by LC - MS/MS methods. Methods：The samples were separated on a Diamonsil Cls（ 150 mmx 4.6 mm, 5 Ixm） column using acetoni- trile and 0.5% formic acid solution as the mobile phase at a flow rate of 0.6 mL · min-1. The column temperature was room temperature. Selective - reaction monitoring （SRM） scanning was employed for quantification with electrospray ion source polarity in positive mode. Results：The linear range determination of arbutin was 1.0 - 150 μg ·mL-1, and the mean recovery at low, middle and high levels were 98.8% ,98.8% 和11 99.7% ,respectively, and the RSDs were all less than 1.1%. Conclusion：The method is simple, accurate and sensitive for the determination of arbutin content in Selaginel- la tamariscina（ Beauv. ） Spring.