纳晶微针的促渗透作用及安全性实验研究

Study on nanocrystalline needles in skin cosmetology

目的探讨纳晶针作用于皮肤后,可在皮肤表面形成开放给药通道,从而促进外用药物经皮渗透和吸收的价值及安全性。方法 (1)扫描电镜观察纳晶针作用后在皮肤表面形成针孔的存在和闭合;(2)以0.125%荧光素钠为模型药物,在荧光显微镜下对比观察纳晶针+荧光素钠和单纯外用荧光素钠后,荧光在大鼠皮肤的分布情况。结果 (1)纳晶针可在皮肤表层形成给药通道,该通道在20 min左右即可闭合;(2)经过纳晶针处理过的大鼠皮肤可见耀眼的荧光(+++);而未经纳晶针处理的大鼠皮肤仅在角质层可见明确的线状荧光(+),其他部位可见微弱荧光(-)。结论纳晶针对外用药物有明显的促进渗透作用,且安全简便。

Objective To prove that skin＇s open channels of administration after using nanocrystalline needles and that the channels can be closed.Methods （1） Nanocrystalline pinholes were observed using scanning electron microscopy.（2）0.125％ NaFI was used as model drug; skin fluorescence distribution was observed using fluorescence microscopy.Results （1） Nanocrystalline needlc could form administration channels in the skin surface,which would be closed in about 20 min.（2） Bright fluoresence （＋ ＋ ＋） was visible in the skin of rats treated with nanocrystalline needles,but in the rat skin without nanocrystalline needles there was only linear fluoresence （＋） or weak fluoresence （-）.Conclusion Nanocrystalline needles obviously promote infiltration of topical drugs.