摘要
从人工老化后的不同发芽率水平的老芒麦干种子中提取基因组DNA,检测表明其可用于老芒麦种质资源的ISSR分析.ISSR标记分析表明,种子老化引起的老芒麦干种子基因组DNA分子片段的变化主要集中在750～2000bp和250～500bp范围,主要表现为DNA大分子片段的消失或颜色逐渐变浅,某些特异DNA片段出现,且其丢失和增加是随机的.
Dry seed genomic DNA was extracted after artificial aging to be used for ISSR-PCR genetic integrity analysis of Elyrnus sibiricus L. germplasm resources. The changes of dry seed genomic DNA fragments of Elymus sibiricus L. caused by seed aging mainly concentrated in 750--2000bp and 250-- 500bp and showed that macromolecular fragments of DNA disappeared and color became shallow gradually. Some specific DNA fragments appeared, and the lost and increase of genomic DNA fragments were random.
出处
《中国草地学报》
CSCD
北大核心
2015年第2期63-67,共5页
Chinese Journal of Grassland
基金
"十二五"农村领域国家科技计划课题"淀粉类(小麦野生材料)种质遗传完整性新技术研究及监测"(2013BAD01B0105)
中国农业科学院科技创新工程(草原研究所2015-01)
中央级公益性科研院所基本科研业务费"种子老化对老芒麦种质遗传完整性的影响"(1610332014020)资助
关键词
老芒麦
种子老化
Elymus sibiricus L
Seed aging
ISSR
