ADSCs对辐射诱发C57小鼠胸腺瘤免疫功能的影响及其机制

Effect of ADSCs on immune function of C57 mice with thymomas induced by irradiation and mechanism

目的:观察脂肪来源干细胞(adipose-derived stem cells,ADSCs)对辐射诱发小鼠胸腺瘤免疫功能的影响及其可能的机制,方法:清洁级C57BL/6小鼠32只,随机分为4组:对照组、照射组、照射+ADSCs组、假照射+ADSCs组,各8只,末次照射后第7天后分别处死4组小鼠,计算小鼠胸腺指数。刀豆素A(ConA)诱导的胸腺淋巴细胞转化率采用MTY法测定,运用流式细胞仪检测胸腺淋巴细胞的细胞周期以及T淋巴细胞亚群的变化。结果:照射+ADSCs组相对于照射组小鼠的胸腺淋巴细胞转化率和胸腺指数显著增高(P<0.05),并且CD4+T淋巴细胞的含量显著增加(P<0.05),CD8+T淋巴细胞明显下降(P<0.05),CD4+/CD8+T淋巴细胞比值显著升高(P<0.05)。结论:ADSCs可能通过调节小鼠胸腺内细胞水平的变化,保护小鼠胸腺细胞的免疫功能。

Objective： To investigate that the effect of adipose-derived stem cells（ADSCs） on immune function of C57 mice with thymomas induced by irradiation, and plausible mechanism. Methods： A total of 32 cleaning degree C57BL/6 mice were divided into four groups： control group of 8 mice with non-irradiation; irradiation group of 8 mice with irradiation; irradi- ation＋ADSCs group of 8 mice, thymoma model mice were injected 0.5 mL ADSCs via tail vein at one day after last irradia- tion; non-irradiation＋ADSCs group of 8 mice with the same ADSCs injection as irradiation＋ADSCs group. Mice in four groups were sacrificed at 7th day after last irradiation, thymus index was measured by weight and thymus lymphocyte transfor mation rate was detected by MTY. Thymus lymphocyte cycle and T lymphocyte subsets were determined by flow cytometry. Results： Compared with irradiation group mice, lymphocyte transformation rate and thymus index was significantly higher in irradiation＋ADSCs group mice. When detected by flow cytometry, CD4^＋ T cell subsets and CD4^＋/CD8^＋ from irradiation＋ ADSCs group mice increased, CD8^＋ T cell subsets from irradiation＋ADSCs group mice was decreased, there were statistical significance （P〈0.05）. Conclusion： ADSCs may regulate the cell levels of mice thymus and protect immune function of mice.