缺氧预适应对体外培养神经元的神经保护作用存在一个时间窗

There is a time window in neuroprotective function of hypoxia preconditioning on external cultured neurons

目的本实验采用体外培养皮质神经元研究缺氧预适应对谷氨酸损伤模型的保护作用,同时探索一定缺氧浓度下最佳保护作用的缺氧时间。方法原代培养神经元,确立谷氨酸损伤模型,对培养至第7天的细胞进行8%氧浓度和不同时间的缺氧预适应及谷氨酸损伤,用MTT法检测细胞的存活率。应用SPSS 17.0进行统计学分析。结果 8%O2缺氧4-8 h,皮质神经元谷氨酸损伤模型实验组与对照组相比,存活率提高,差异有统计学意义（P〈0.05）。结论 8%O2缺氧4-8 h对谷氨酸损伤模型中的原代体外培养皮质神经元具有保护作用,该保护作用至少可持续6 h。

Objective This experiment aims at studying the protective function of Hypoxia Preconditioning for neurons cultured in vitro,and exploring the best oxygen concentration,as well as the time of hypoxia. Methods The primary cortical neurons achieved from neonatal Wistar rats born in 24 hours. The growth curve of cells and glutamate injury model were determinate by MTT. The cells that cultured to the 7th day,were injured by glutamic acid after hypoxia preconditioning with 8% oxygen concentration and different duration. Conduct T-test and anova to survival rate of the cells with SPSS 17. 0.Results Under the circumstance of 8% O2 with 4 - 8 hours oxygen deficit,the survival rate of cortical neuron model increased compared with the control group. Conclusion As for cortical neurons,the protective action takes place under the circumstance of 8% O2,4 - 8 hours oxygen deficit. Protective action due to Hypoxia Preconditioning can be sustained for at least 6 hours.