摘要
为研究硫酸酯化酵母β-葡聚糖对流感病毒血凝抑制作用及对mRNA转录的影响,以猪流感病毒A/tianjin/1/TJ2(H1N1)感染MDCK细胞为模型,采用血凝抑制试验(HI)及SYBR Green Real-time PCR方法,探索硫酸酯化酵母β-葡聚糖对流感病毒血凝抑制作用及其对mRNA转录影响。结果显示,硫酸酯化酵母β-葡聚糖在浓度超过1.25 mg/m L时,能对猪流感病毒血凝产生抑制作用,且在5 mg/m L时对猪流感病毒mRNA转录具有抑制作用。因此,硫酸酯化酵母β-葡聚糖具有抑制流感病毒在MDCK细胞复制的作用,其作用机制可能与抑制流感病毒HA靶点及影响mRNA转录有关。
To study the effect of sulfated yeast β-glucan on mRNA transcription and hemagglutination of H 1N1 swine influenza virus in vitro,influenza virus infected MDCK was taken as model ,SYBR Green Real-time PCR and HI test was replication adopted to measure the effect of sulfated yeast β-glucan on mRNA transcription and hemag-glutination .The results showed that the sulfated β-glucan could inhabit the hemagglutination at the concentration of over 1.25 mg/mL,while it could inhibit influenza virus′s mRNA transcription at the concentration of 5 mg/mL.Sul-fated yeast β-glucan had the ability to inhabit reproduction of H 1N1 SIV in vitro,and the ability to inhabit hemag-glutination and mRNA transcription may be targets of sulfated yeast β-glucan to anti-SIV.
出处
《华北农学报》
CSCD
北大核心
2015年第1期15-18,共4页
Acta Agriculturae Boreali-Sinica
基金
天津市应用基础及前沿技术研究计划项目(12JCYBJC20100)
