摘要
为了分离克隆与植物互作的大丽轮枝菌致病相关蛋白基因，利用 SMART 技术构建了大丽轮枝菌酵母双杂交 cDNA 文库。结果表明，构建的文库滴度为5.2×107 cfu/mL，库容为3.9×107 cfu；文库 cDNA 插入片段长度主要分布在0.5~2.0 kb，平均为1 kb；文库重组率为96%。表明文库质量较好，为筛选与植物互作的大丽轮枝菌致病蛋白基因奠定了基础。
To seek out the V. dahliae proteins involved in the interaction of plant with V. dahliae,a yeast two- hybrid library of V. dahliae was constructed using SMART technique. Results showed that the titer of cDNA library was 5.2 × 107 cfu/mL, the library contained 3.9 × 107 cfu independent clones, the size distribution of insert fragments ranged from 0.5 -2.0 kb,and the recombination rate was about 96%. These data demonstrated that the library could meet the requirements of standard cDNA library,which laid a foundation for screening the interaction proteins from V. dahliae.
出处
《华北农学报》
CSCD
北大核心
2015年第2期51-54,共4页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金项目(31440076
31272168)
关键词
黄萎病
大丽轮枝菌
酵母双杂交文库
SMART技术
VerticiUium wilt
Verticillium dahliae Kleb.
Yeast two-hybrid cDNA library
SMART technique
