利用DH群体构建大白菜分子遗传图谱

Constructing Genetic Linkage Map of Chinese Cabbage with Double Haploid( DH) Population

构建大白菜分子遗传图谱,为大白菜干烧心QTL定位和分析奠定基础。以大白菜感病品种B120和大白菜抗病品种黑227为亲本建立的DH系为作图群体,基于所筛选出的74对In Del标记和37对SSR标记构建分子遗传图谱。利用Join Map 4.0软件,初步构建了一张覆盖基因组长度为1 004.7 c M、平均图距为9.30 c M的大白菜遗传连锁图,该图谱包含12个连锁群、108个标记位点。该图谱能有效地用于大白菜干烧心QTL定位。

A molecular genetic map of Chinese cabbage was constructed,whose aim that the location and analy-sis of QTL of tipburn in Chinese. The map was constructed based on 74 InDels markers and 37 SSRs markers using DNA samples extracted from a doubled haploid population obtained through microspore culture from the F1 between two homozygous parents：B120 and Black 227. By using JoinMap 4. 0 software analysis,the map consisted 12 linkage groups,included 108 genetic markers and covered 1 004. 7 cM with an average distance of 9. 30 cM. The map would be used effectively to QTL location of tipburn in Chinese cabbage.