玉米须、马齿苋提取物对小鼠抗疲劳作用及配伍协同研究

Comparison of the Anti-fatigue Effects of Zea mays and Portulaca oleracea Unilateral and Compatibility in Mice

目的研究玉米须、马齿苋提取物及二者配伍的抗疲劳作用及其机制。方法将小鼠随机分成正常对照组、玉米须提取物组、马齿苋提取物组、玉米须马齿苋提取物(1:2)配伍组,以生药为500mg/kg·d-1,正常对照组给予同等剂量的生理盐水连续给药30d,测定负重游泳时间、血清尿素氮和肝糖原水平。结果与正常组比较,各组均可显著延长小鼠游泳耗竭时间(P<0.05或P<0.01),提高肝糖原的含量(P<0.05或P<0.01),且配伍组抗疲劳作用优于单一提取物组(P<0.05);但各组对小鼠运动后血清尿素氮水平均无明显影响(P>0.05)。结论玉米须、马齿苋提取物配伍对小鼠有抗疲劳作用,且两者配伍抗疲劳作用更显著。

Objective The purpose of this study aimed to investigate the anti-fatigue effect of Z. mays and P. oleracea and compatibility. Methods Mice were randomly divided into blank control group, Z. mays extract group, P. oleracea extract group, Z. ays, P. oleracea extract （1：2） compatibility group to crude drug was 500mg/kg.d^-1, control group was given the same dose of saline administered continuously for 30 days, swimming time was measured in serum urea nitrogen and liver glycogen levels. Results with the blank group, each group can significantly prolong the swimming depletion time （P〈0.05 or P〈0.01）, increase glycogen content （P〈0.05 or P〈0.01 ）, and the compatibility group fatigue superior to single extract group （P〈0.05）;but each group had no significant effect on serum urea nitrogen levels in mice after movement （P〉0.05）. Conclusion Zea mays and Portulaca oleracea extracts have significant anti-fatigue effects in mice. Both share can obtain a better result, better than used alone.