响应面法优化诺丽多糖提取工艺

Optimization of Extraction Conditions of Polysaccharide from Morinda citrifolia by Response Surface Methodology

目的采用响应面法对诺丽多糖的提取工艺进行优化。方法在单因素实验的基础上,以提取温度、液料比、提取时间为自变量,总多糖含量为响应值,研究各因素及其交互作用对总多糖得率的影响。结果最优工艺条件为,液料比为1∶40g·m L-1,提取温度为100℃,浸提时间为4h,提取实际值为9.677mg·g-1。结论采用响应面法优化诺丽总多糖提取工艺是合理可行的,实验结果将为诺丽多糖新产品研发提供物质基础。

Objective Response surface methodology was used to optimize the extraction conditions of polysaceharide from Morinda citrifolia. Methods Based on one factor test, the individual and interactive effects of ethanol concentration, material/liquid ratio, extracting temperature and extracting time on the yield of polysaccharide were studied. Results The experiment indicated that the optimal conditions include material/liquid ratio 1：40, extraction temperature 100℃ and extraction time 4h. Under these conditions,the yieht of polysaccharide was 9. 677mg ·g^-1. Conclusion This exhibits the good feasibility of Response surface methodology for optimizing extracting process of polysaccharide from Morinda citrifolia.