郁金香‘上农早霞’花色变异相关基因的信息分析

Analysis of Differentially Expressed Genes in Petals of a Mutant Tulipa fosteriana 'Shangnong zaoxia' and its' Parent Line

郁金香(Tulipafosteriana)红花品种‘上农早霞’是粉花品种‘Albert heijn’的突变种。为探讨两者花色差异的原因,采用cDNA-AFLP技术分析了2品种花瓣中基因的表达差异,共获得371条差异表达的转录衍生片断(transcript-derived fragment,TDFs)。通过BLAST比对,75条ESTs与已知蛋白具较高同源性(GenBank登陆号:JZ349173-JZ349247)。利用COG、GO和KEGG对其功能和参与的生化途径进行注解,表明这些蛋白具有催化活性、转移酶活性、结合活性等;参与糖类和脂类代谢、信号转导、产物转运等26个生物学过程;涉及新陈代谢、蛋白加工、RNA转运等15个生化途径。选择4个可能与花色变异相关的ESTs(分别编码反转录转座子蛋白、谷胱苷肽S转移酶、类黄酮3’羟化酶以及类胡萝卜素异构酶),应用Real-time PCR技术分析它们在‘上农早霞’和‘Albert heijn’之间的表达差异。结果显示在花朵发育过程中,反转录转座子蛋白以及谷胱苷肽S转移酶基因在‘上农早霞’中的表达水平一直高于‘Albert heijn’;类黄酮3’羟化酶基因在‘上农早霞’中几乎没有表达;类胡萝卜素异构酶基因在‘上农早霞’中的表达水平显著低于‘Albert heijn’,由此推测这4个基因或其所在的生化途径与花色变化紧密相关。本研究结果可为进一步分析‘上农早霞’花色变异机理提供参考,为后续花色变化相关基因的克隆提供基础资料。

The red-flowered cultivar Tulipafosteriana ‘Shangnong zaoxia＇was bred from an imported pink-flowered cultivar‘Albert heijn＇.To analyze the possible reason for flower color deviation,cDNAAFLP technique was employed to investigate the gene expression differences in petals of fully opened flowers between these two cultivars.Among the obtained 371transcript-derived fragments（TDFs）,seventy-five ESTs had their homologous sequences（GenBank Accession No.JZ349173-JZ349247）.COG,GO and KEGG annotations demonstrated that these genes had various molecular functions such as catalytic activity,transferase activity,and binding activity.These genes were involved in 26 biological processes such as carbohydrate,lipid metabolism,and signal transduction,and 15 pathways such as metabolic pathways,protein processing,and RNA transport.Furthermore,the expression profiles of four ESTs（retrotransposon protein,glutathione-s-transferase,flavonoid 3＇-hydroxylase,and carotenoid isomerase）,which were supposed to be related to pigmentation,were analyzed during flower development in two cultivars by realtime PCR.The results showed that retrotransposon protein and glutathione-S-transferase genes expressed at significantly higher level in ‘Shangnong zaoxia＇than those in ‘Albert heijn＇,flavonoid 3＇-hydroxylase gene hardly expressed in ‘Shangnong zaoxia＇,and carotenoid isomerase gene expressed at significantly lower level in‘Shangnong zaoxia＇.The results suggested that these genes and/or their involved biological processes were closely related to the flower color deviation in ‘Shangnong zaoxia＇.These findings will provide references for demonstrating the reasons for flower color deviation in‘Shangnong zaoxia＇and the cloning of the pigmentation-related genes in tulip.