ABCA1基因M883I多态性与子痫前期发病的关联

M883I Polymorphism of ABCA1 Gene is Associated with the Pathogenesis of Preeclampsia

探讨ABCA1基因M883I多态性在福建汉族女性中的分布特点及其与子痫前期患者体内ABCA1蛋白表达、血脂水平的关联性。研究对象400例,包括正常对照组、子痫前期病例组各200例。采用PCR-RFLP法测定ABCA1基因外显子区M883I在病例组及对照组中的多态性分布特点,ELISA法测定ABCA1蛋白表达水平,并检测血脂水平。结果:ABCA1基因M883I多态性对子痫前期的发病率无显著影响,但子痫前期患者中MI+II基因型血清ABCA1蛋白浓度及外周血HDL-C、Apo A1均明显高于MM基因型,差异显著(P<0.05)。结果表明:ABCA1基因M883I在福建汉族女性中,I等位基因可提高血清中ABCA1蛋白的表达,使血液中HDL-C及Apo A1水平升高,从而降低了子痫前期合并血脂代谢紊乱的风险性。

The paper evaluated the association between single nucleotide polymorphism （SNP） of M883I of ABCA1 gene and preeclampsia, ABCA1 protein expression or the level of serum lipids in Fujian Han women in Chinese. Methods： The total number of pregnant Han Chinese women enrolled is 400. The subjects consisted of 200 controls and 200 patients with preeclampsia. SNP M883I at ABCA1 gene was determined by using PCR-RFLP. The level of serum lipids and ABCA1 protein expression was measured by ELISA and Clinical testing. The results showed that M883I polymorphism ofABCA1 gene had no significant effect on the incidence ofpreeclampsia. Among patients with preeclampsia, the expression of ABCA1 protein and the concentration of HDL-C and ApoA1 were significantly higher in those with genotype MI＋II than in those with genotype MM. The differences were significant （P〈0.05）. Conclusion： At locus M883I ofABCA1 gene, allele I could be reducing the risk ofpreeclampsia complicated with metabolic disorder of lipid through elevating the levels of HDL-C and ApoA1 in blood.