辛伐他汀在实验性种植体周围炎中应用研究

Analysis of simvastatin in experimental peri-implantitis

目的探讨辛伐他汀在种植体周围炎骨缺损区应用对种植体骨再结合的影响。方法本研究于2011年6月至2012年2月在哈尔滨医科大学动物实验中心完成。选取4只成年杂种犬,全麻下拔除双侧第一、二、三前磨牙,即刻植入种植体24颗,植入12周确定骨结合后,丝线栓结法建立实验性动物口腔种植体周围炎模型。随机进行分组治疗。(1)空白组(6颗种植体):单纯去除种植体周围炎性肉芽组织,骨缺损区不填入任何材料;(2)实验组(9颗种植体):去除种植体周围炎性肉芽组织,骨缺损区填入骨粉+生物膜+局部注射辛伐他汀(3.0 mg/kg·d);(3)对照组(9颗种植体):去除种植体周围炎性肉芽组织,骨缺损周围填入骨粉+生物膜+局部注射等量空白溶剂。3组术后龈缘内均应用盐酸米诺环素软膏2周(1次/周),实验组和对照组局部注射5 d(1次/d)。术后16周处死动物,行大体观察、缺损区重建骨量测量、电镜观测。结果局部注射辛伐他汀实验组骨重建区骨密度较对照组高,实验组骨重建区骨再结合强度较对照组高,差异有统计学意义(P<0.05)。结论局部注射辛伐他汀可促进骨生长。

Objective To study the impac! of simvastatin on peri-implantitis osseointegration. Methods Select 4 adult clogs and extract bilateral premolars 1, 2 and 3 under general anesthesia; 24 implants was performed immediately in each dog; 12 weeks after surgery, silk knot was used to establish the peri-implantitis model, and they were randomly di- vided and treated with： （ 1 ） simple removal of inflammatory tissue around implant （blank group ） ; （2） debridement plus absorbable membrane associated with bone graft and local injection of simvastatin （3.0 mg/kg-d ）（experiment group ） ; （3） debridement plus absorbable membrane associated with bone graft and local injection of blank solvent （control group）. They were killed after 16 weeks. Naked eye and Scanning electron microscopy was used to observe and measure bone re- construction of defect location. Results There was significant difference in the expressions of bone density and bone strength between experiment group and control group （P 〈 0.05）. Conclusions Simvastatin can improve bone forma- tion.