神经生长因子受体阳性人食管鳞癌细胞对紫杉醇的耐药性研究

Resistance of human esophageal squamous carcinoma cells with positive nerve growth factor receptor expression to paclitaxel

目的 探讨神经生长因子受体阳性（NGFR＋）人食管鳞癌细胞株EC9706对紫杉醇的耐药性.方法 采用噻唑蓝法药物敏感试验测定NGFR＋ EC9706细胞的耐药指数,采用小鼠致瘤实验模型评估紫杉醇作用下的NGFR＋ EC9706细胞与未分选EC9706细胞异种移植肿瘤生长的差异.结果 NGFR＋EC9706细胞的耐药指数为2.03,NGFR＋ EC9706细胞致瘤的紫杉醇组与0.9％氯化钠溶液组瘤重无明显差异（P＞0.05）,实体瘤大小差别不明显.而未分选EC9706细胞致瘤的紫杉醇组与0.9％氯化钠溶液组比较瘤重明显减小[（0.5±0.4）g比（1.9±1.0）g],差异有统计学意义（P＜0.05）,实体瘤也明显减小.未分选EC9706细胞致瘤的紫杉醇组较NGFR＋ EC9706细胞致瘤的紫杉醇组瘤重明显减小[（0.5±0.4）g比（1.3±0.4）g],差异有统计学意义（P＜0.05）.结论 人食管鳞癌细胞中的NGFR＋ EC9706细胞对紫杉醇具有一定的耐药性.

Objective To explore the resistance of human esophageal squamous carcinoma cell line EC9706 with positive nerve growth factor receptor （NGFR） expression to paclitaxel.Methods Resistance index of NGFR ＋ EC9706 was measured by four methyl tetrazolium methods in vitro.The growth of NGFR ＋ EC9706 and non-sorted EC9706 was assessed by transplantation tumor model in nude mice.Results The resistance index of NGFR ＋ EC9706 was 2.03.In transplantation tumor model induced by NGFR ＋ EC9706,the tumor weight showed no significant difference between paclitaxel group and saline group;in transplantation tumor model induced by nonsorted EC9706,the tumor weight was significantly reduced in paclitaxel group compared with that in saline group [（0.5 ± 0.4） g vs （1.9 ± 1.0） g,P ＜ 0.05].In addition,the tumor weigh was slighter in non-sorted EC9706 induced transplantation tumor than that in NGFR ＋ EC9706 induced transplantation tumor [（0.5 ± 0.4） g vs （1.3 ± 0.4） g,P ＜ 0.05].Conclusion NGFR ＋ EC9706 cells are resistant to paclitaxel.