响应面法优化微藻Chlorella sp.U4341油脂产率的研究

Research on Optimization of Lipid Productivity by Chlorella sp.U4341 with Response Surface Method

为了提高微藻Chlorella sp.U4341的油脂产率,采用响应面法对异养培养条件进行优化研究.通过单因素试验筛选出促进Chlorella sp.U4341油脂产率的最佳碳源、氮源分别为葡萄糖、Na NO3.利用Plackett-Burman试验筛选出显著影响Chlorella sp.U4341油脂产率的三个因子,通过最陡爬坡试验逼近最大油脂产率区域,运用中心组合试验及响应面分析确定了三个因子的最佳浓度:葡萄糖为24.81 g/L,Na NO3为1.68 g/L,K2HPO4·3H2O为0.58 g/L.优化后的条件培养微藻Chlorella sp.U4341的油脂产率为273.34 mg/L·d,比优化前提高了58%.

To increase the lipid productivity of microalgae Chlorella sp.U4341 ,optimization of heterotrophic cul-tivation condition is carried out by response surface method.The optimal carbon and nitrogen sources for Chlorella sp.U4341 are glucose and sodium nitrate,respectively.Three most important influencing factors for lipid produc-tivity of Chlorella sp.U4341 are screened via Plackett -Burman experiment.Subsequently,the steepest ascent ex-periment is used to guide key factors to approach the maximum lipid productivity region.Optimal concentrations of the three influencing factors are confirmed by central composite design and response surface analysis.The re-sults are 24.81 g/L of glucose,1 .68 g/L of sodium nitrate and 0.58 g/L of dipotassium phosphate trihydrate. Cultivated with the optimized condition,the lipid productivity of Chlorella sp.U4341 reaches 273.34 mg/L·d, increased by 58% compared with the original cultivation condition.