高效液相色谱法测定止咳定喘丸中苦杏仁苷含量

Determination of Amygdalin Content in Zhike Dingchuan Pills by HPLC

目的：建立测定止咳定喘丸中苦杏仁苷含量的高效液相色谱法。方法色谱柱为 Agela Venusil MP C18柱（250 mm ×4.6 mm，5μm），流动相为甲醇－水（20：80），流速为1.0 mL/min，检测波长为210 nm，柱温室温。结果苦杏仁苷进样量在0.0530~0.8480μg （ r=0.9999）范围内与峰面积积分值具有良好线性关系，平均加样回收率为98.64%，RSD为1.11%（ n=6）。结论该法简便、灵敏、准确可靠，可用于止咳定喘丸中苦杏仁苷的含量测定。

Objective To establish a HPLC method for the determination of the amygdalin content in Zhike Dingchuan Pills. Methods The Agela Venusil MP C18 column （ 250 mm × 4. 6 mm, 5 μm ） was used with the mobile phase of methanol-water （ 20 ：80 ） , the flow rate was 1. 0 mL/min, the detection wavelength was 210 nm and the column temperature was the room temperature. Results The sam-ple size of amygdalin in the range of 0. 053 0-0. 848 0 μg （ r=0. 999 9 ） showed a good linear relationship with the peak area. The average recovery rate was 98. 64% with RSD=1. 10% （ n=6 ） . Conclusion This method is simple, sensitive and accurate, and can be used for the determination of the amygdalin content in Zhike Dingchuan Pills.