猪附红细胞体病三种诊断方法的比较

Comparison of Three Diagnostic Methods of Eperythrozoonsis

采集了甘肃省内3个规模猪场的血液样品300份,分别用显微镜检测、ELISA、PCR方法进行比对实验,比较三种检测方法的特异性和敏感性。结果表明,显微镜检测存在较高的假阳性,PCR的特异性和敏感性最好,分别达到93.3%和95.7%。进行监测时,建议选择镜检和PCR的联合诊断方法。

In this study, 300 porcine blood samples were collected from 3 large--scale pig farms in Gansu province to diagnose eperythrozoonsis by microscopy, ELISA and PCR methods, and then compare the specificity and sensibility of these methods. The results showed that sensitivity of the PCR was the best （93.3%）, the sensitivity of ELISA and microscopy were 86. 7% and 76.7M respectively. Besides, the specificity of the PCR was also the best （95.7%）, while the specificity of ELISA and microscopy were 90.0% and 85.7% respectively. So this experiment can provide the basis for the establishment of detection scheme of eperythrozoonsis in large--scale pig farm.