HPLC法测定倍他米松尿素乳膏中倍他米松和羟苯乙酯的含量

Determination of Beclomethasone and Ethylparaben in Beclometasone and Urea Cream by HPLC

目的建立测定倍他米松尿素乳膏中倍他米松和羟苯乙酯含量的方法。方法色谱柱：PLATISILTMODS（250 mm×4.6 mm,5μm）,流动相：乙腈-水（30∶70）,检测波长：240 nm,流速：1.0 ml·min-1,柱温：45℃。结果倍他米松、羟苯乙酯分别在3.94~23.64μg·ml-1（r=0.9999）、4.78~28.68μg·ml-1（r=1.0000）范围内线性关系良好;回收率、RSD分别为倍他米松99.7%、0.92%,羟苯乙酯100.4%、0.40%。结论本方法简便、准确、重复性好,可用于该制剂中倍他米松和羟苯乙酯的含量测定。

Objective To establish an HPLC method for content determination of beclomethasone and ethylparaben in Beclometasone and Urea Cream.Methods HPLC analysis was carried out on Diamonsil C18column（250 mm × 4.6 mm,5 μm） and with C2H3N-H2O（30∶ 70） as the mobile phase.The detection wavelength was 240 nm,the flow rate was 1.0 ml·min-1and the column temperature was 45 ℃.Results Beclomethasone showed a good linear relationship in the range of 3.94-23.64 μg·ml-1（r = 0.9999）,and ethylparaben in the range of 4.78-28.68 μg·ml-1（r = 1.0000）.The recovery and RSD of beclomethasone and ethylparaben in Beclometasone and Urea Cream were 99.7%,0.92%,100.4% and 0.40%,respectively.Conclusion This method is simple,accurate and repeatable,which is suitable for content determination of beclomethasone and ethylparaben in Beclometasone and Urea Cream.