香樟叶对菜青虫拒食的活性成分研究

Study on Antifeeding Active Constituent of Camphor Leaves to Pieris rapae

用气质色谱法分析了香樟叶中的主要挥发性成分；用叶碟法测定了香樟叶不同试剂提取物对菜青虫的拒食作用、菜青虫对不同剂量的乙醇提取物拒食反应和香樟叶中主要成分芳樟醇和樟脑对菜青虫的拒食活性。结果表明，香樟叶中的主要挥发性成分分别为芳樟醇和樟脑25．013％、桉油醇13．5723％。不同提取物的生测结果是乙醇提取物对菜青虫的拒食作用强于甲醇和乙酸乙酯提取物，拒食反应指数分别为97．98、34．33和39．71；乙醇提取物的不同剂量测定结果表明，使用剂量越大对菜青虫的拒食作用越强；其主要成分芳樟醇和樟脑的拒食反应指数分别为89．36和39．88，说明香樟叶的拒食活性主要是芳樟醇和樟脑的作用，其它成分或有协同。

The main volatilize component from Camphor leaves was analyzed by GC/MS. The extracts from Camphor leaves using different solvents to the effect of anti-feeding,the response of anti - feeding to extracts of different doses of ethanol ,the linalool and Camphor eamphom of main components from Camphor leaves to activity of anti-feeding to Pieris rapae were measured by leaf disc test. The results show that the main volatilize components from Camphor leaves are the linalool and Camphor camphora taking 25. 013% and euealyptol taking 13. 572% respectively. Bioassay results of different extracts show that the effect of ethanol to anti-feedlng of Pierls rapae is stronger than that of methanol and ethyl acetate; the indexes of anti-feeding response are 97.98,34.33, and 39.71 respectively. The results of extracts of different doses ethanol show that the large the doses, the stronger the anti-feeding. The indexes of anti-feedlng response about the linalool and Camphor campbera from its main components are 89.36 and 39.88 ,it indicated that the anti-feedlng activity of Camphor leaves is mainly the effect of linalool and Camphor camphora, and other components maybe have synergistic reaction.