金银花枝叶中多糖的组成测定及抗油脂氧化研究

Component Determination and Antioxidant Activity to Lipin of Polysaccharide from Lonicera Japonica branches

用超声波辅助水提取金银花枝叶中的粗多糖，醇沉除色素及蛋白后得到多糖纯化液，使用簿层色谱、红外光谱和紫外光谱鉴定其组成，研究多糖纯化液对油脂氧化的抑制作用。结果表明，金银花枝叶粗多糖得率为13．20％，多糖纯化后葡萄糖含量为70．30％，多糖纯化液经HC1水解后含甘露糖及麦芽糖成分，多糖纯化液对油脂氧化有很好的抑制作用。

Polysaecharldes （PS） were extracted from Lonicera Japonica branches by water-extraction and ethanol precipitation with ultrasonic assistance. The crude polysaccharide was puP.fled through decolorafion ~ith petroleum ether and deprotelnized by Sevag method. Purified polysaccharlde was determinated by Thin Layer Chromatography （TLC）, Infrared Spectroscopy （IR） and Ultraviolet spectroscopy （UV）. The antioxidant activities of purified polysaccharide to lipin were investigated. The results showed that yield of crude polysaccharides was 13.20% and glucose component was 70. 30% in purified polysaccharidos. Mannose and Maltose were detected in purified polysaccharidos after HC1 hydrolysis. Purified polysaccharide exhibited good inhibitory effect to peroxidation of oils and fats, although its antioxidant activity was weak compare to that of Vc and citric acid.