不同生境对蒲公英体内三种黄酮含量的影响

Determination of Three Flavonoids of Taraxacum Mongolicum from Different Habitats

本试验建立蒲公英中三种黄酮类物质含量的HPLC测定方法，探讨旱生、湿生两种不同生境对蒲公英体内黄酮含量的影响。采用Inertsil ODS-SP色谱柱（150mm×4．6mm，5μm），柱温为30℃，流速为1．0mL／min，进样量为10μL，等度洗脱。发现旱生全草芦丁、木犀草素、槲皮素3种黄酮类含量均显著高于湿生全草。旱生叶、花的芦丁含量显著高于湿生叶和花，旱生花的木犀草素含量显著高于湿生，其它各器官间差异不显著；而除花葶外，旱生各器官槲皮素的含量均显著高于湿生。同在旱生、湿生生境下，芦丁含量均以叶中最高，而木犀草素及槲皮素则以花中含量最高。

The research established a method to detect the content of 3 of ilavonoids of Taraxacum mongolicum. Further more, discussed the effect of different habitats on the flavonoids content in T. mongolicum. The separation was performed on Iinertsil ODS-SP column （150 mm ×4.6,5 μm）. The column temperature was 30 ℃. The flow rate was 1.0 mL/ rain. The injection volume was 10μL. Isocratic elution. The content of three kinds d flavonoids （ mtin,lutcolin and quercetin） of the whole plant of T. mongolicum in the xeric environment were higher than the wet. In two habitats,rutin content of leaves and flowers of xeric was higher than the wet,and lutcolin content of flowers of xeric was more than the wet, but there is no significant differences among other organs. Quercetin content of different xeric organs,except the scape, was higher compare to the wet one. In the same habitats,the rutin content of leaves was the most among different organs, but the content of luteolin and quereetin of flowers were higher than others.