摘要
以黍稷麸皮Cimi2为研究对象,比较了四种多酚提取方法的效果及提取物清除自由基的能力。从总酚来看,用乙醇浸提法、超声助溶法、酶解法以及超声与酶结合法分别得到225.77、192.23、231.01、221.15rag没食子酸/100g样品。四种方法中得到的结合酚和自由酚比例为49:51、29:71,31:69、28:72。对9个品种的麸皮提取物的体外抗氧化能力检测结果表明,黍稷麸皮酚酸对DPPH、超氧阴离子、羟自由基都有明显的清除作用。其中zigangmi清除DPPH自由基能力和体外总抗氧化能力最强最强,Cimi2的清除羟自由基能力最强。
The bran of prose millet cultivars were analyzed for their phenolic content and antioxidant activities. Four extraction methods, including ethanol solvent, ultrasonlc-assisted ethanol extractlon、enzymolysis,ultrasonic-enzymolysis method,were compared for their efficiency of phenolic content of the Cimi2 bran. The total phenolic content were 225. 77 、192. 23 ,231. 01,221.15 rag/100 g respectively. The ratios of bound phenolic to free phenolic were 49: 51,29: 71,31 : 69、28:72 for four extraction methods respectively. Total phenolics from prose millet bran has a scavenging activity on DPPH、 O2 and · OH radicals. The highest DPPH scavenging ability and total antioxidant capacity was found in zigangml bran. The highest hydroxyl radical scavenging ability was found in Cimi2 bran.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2014年第A01期110-113,82,共5页
Natural Product Research and Development
基金
国家现代农业产业技术体系一国家谷子糜子产业技术体系(CARS-07-12.5-A12)
山西省留学基金(2014-101)
山西省国际合作项目(2014081059)
关键词
黍稷
自由酚
结合酚
提取方法
抗氧化能力
Panicum miliaceum L. ,free phenolic
bound phenolic
extraction methods
antioxldant activity