大叶小檗体内抗肿瘤活性的研究

Study on Antitumor Activity in vivo of Berberis amurensis

目的：研究大叶小檗75％乙醇提取物及总生物碱对如荷瘤小鼠肿瘤的抑制作用，为进一步开发其药用价值提供依据。方法：按照标准方法给小鼠接种H恐荷瘤细胞，随机分为7组，分别腹腔注射给予不同剂量的大叶小檗75％乙醇提取物、大叶小檗总生物碱、生理盐水、环磷酰胺，12d后分别摘取肿瘤块、脾、胸腺称重并计算肿瘤抑制率，同时分别计算脾和胸腺指数。结果：给药组瘤重明显低于阴性对照组，且给药组与阴性组对比均有极显著性差异（P〈0．01），75％乙醇提取物及总生物碱在浓度为500mg／kg时抑瘤率均大于60％，当两者浓度达到1000mg/kg时，两者抑瘤率相同，且效果最佳，可达到73．83％，阴性组免疫器官指数与空白组对比均有极显著性差异（P〈0．01），大叶小檗75％乙醇提取物及总生物碱在高低剂量组时，胸腺指数均高于阳性组，低于阴性组，脾指数均高于阳性组，75％乙醇提取物及总生物碱在剂量为1000mg／kg时对荷瘤小鼠免疫器官有影响，给药组指数与阳性对照组、空白组也均有极显著性差异（P〈0．01）。结论：大叶小檗75％乙醇提取物及总生物碱均能较好的抑制H22荷瘤小鼠肝癌在其体内的生长。

To study the amur barberry root 75% ethanol extract and total alkaloid of H22, mice tumor-burdened bone tumor inhibition and provide the basis for further development of its medical value. According to the standard method for vaccination H22, mice tumor-burdened bone ceils, randomly divided into 7 groups, respectively given different doses of a- mur barberry root produced by intrapefitoneal injection of 75% ethanol extract, amur barberry root total alkaloid, physiological saline,cyclophosphamide,gather the tumor after 12 d, block, spleen and thymus weight and calculate the tumur inhibition rate,spleen and thymus index are calculated separately, and at the same time. Give medicine group, tumor weight significantly loss than the negative control group and drug group compared with negative groups had extremely significant differenee（P 〈0.01 ） ,75% ethanol extract and total alkaloids in the concentration ofS00 mg/kg YiLiuLv were greater than 60% ,when the concentration of 1000 mg/kg,beth YiLiuLv are the same,and the effect is best,can reach 73.83% ,negative immune organ index and the blank group comparison has the extremely significant difference（ P 〈 0. 01） ,amur barberry root 75% ethanol extract and total alkaloids in high and low dose group,the thymus index were higher than in positive group, lower than that of negative group, spleen index were higher than in positive group,75% ethanol extract and total alkaloids in the dose of 1000 mg/kg to influence a tumor-burdened mice immune organs,to medicine group index,the blank group and positive control group also had extremely significant difference（P 〈 0.01 ）. The amur barberry root 75% ethanol extract and total alkaloids could better inhibiting H~, tumor-burdened in mice liver cancer in its growth.