尾叶香茶菜总二萜的抗炎活性及其LC—MS成分分析

Anti-inflammatory Activity of Total Diterpenoids in Rabdosia excisa and Its LC-MS Analysis

本文测定了尾叶香茶菜总二萜抗炎活性，并进行初步成分分析。使用小鼠腹腔巨噬细胞RAW264．7考察二萜类成分对肿瘤坏死因子（TNF-α）表达的影响以及对二甲苯诱导的小鼠耳肿胀的抑制作用。用液相色谱-质谱联用技术鉴定出总二萜中的6种成分。结果表明，尾叶香茶菜总二萜和尾叶香茶菜丙素（KA，100pg／mL）．可明显降低脂多糖（LPS）诱导的巨噬细胞炎症因子表达，抑制炎症反应。与空白对照组比较，乙醇提取物、总二萜和尾叶香茶菜丙素的高剂量组小鼠耳肿胀明显降低，低剂量组也有降低趋势，其中乙醇提取物低剂量组的抗肿胀作用接近吲哚美辛（Indo，25mg／kg）。

The antl-inflammatory activity and constituents of total diterpenoids in Rabdosia exeisa were evaluated. Antiinflammatory action of total diterpenoids was carded out by the macrophage RAW264.7 cells and ear edema induced by dimethylhenzene in mice. The chemical constituents of total diterpenoids fraction were identified by liquid chromatography-mass spectrometry （LC-MS）. The results indicated that total diterpenoids and kamebakanrin （100μg/mL） could reduce significantly lipopolysaccharide （LPS）-induced macrophage inflammatory cytokine. Compared with control group, the high dose groups significantly reduced the swelling induced by dimethylbenzene in mice. The low dose group had the tendency of inhibition,and ethanol extract （low dose, 100 mg/kg） showed the inhibition closing to indomethacin （Indo, 25 mg/kg）.