HPLC法检测玛咖中芥子油苷成分的研究

Research on High Performance Liquid Chromatography（HPLC） for Glucosinolates in Lepidium meyenii（MACA）

建立玛咖中芥子油苷成分的高效液相检测方法。采用80％甲醇溶液萃取、过DEAE-Sephadex A-25交换柱及硫酸酯酶酶解处理获得玛咖中芥子油苷样品，以苄基芥子油苷为外标对照品，C18色谱柱，流动相A为0．05％四甲基氯化铵水溶液，流动相B为0．05％四甲基氯化铵、20％乙腈的水溶液，梯度洗脱，检测波长229nm，对玛咖芥子油苷含量进行检测。结果表明，玛咖芥子油苷的标准曲线在0．0179—0．2684mg／mL浓度范围内呈现良好的线性关系（R2=0．9999），精密度相对标准偏差为0．052％，不同浓度加标回收率为89．2％～93．0％，最低检测线可达到1μg／kg。实验证实：该方法简单、稳定、灵敏度高，可作为玛咖芥子油苷的常规检测方法。

This study aimed to establish one smiple and fast analytical method for detecting glucosinolate in Maca by high performance liquid chromatography（HPLC）. Maca powder was extracted with 80% methanol, then the extractions were absorbed onto a column packed with DEAE-Sephadex A-25 ,followed by enzymatic hydrolysis with sulfatase, after this step we obtained the sample to be tested. The chromatographic method was carried out on a C18 column using the 0.05% Tetra-methylammonlum chloride aqueous solution as the mobile phase A and 0.05% Tetra-methylammonium chloride aqueous solution（ containing 20% acetonitrile, V/V）as B, Benzyl glucosinolate as standard reference material. The flow rate was 1 mL/min on the gradient elution. The monitoring wavelength was at 229 nm. Results showed that linear ranges of glucosinolate was between 0. 0179-0. 2584 mg/mL（R2 = 0.9999）. The RSD of precision was 0. 052%. The sample recovery rate in different concentrations was 89.2% -93.0%. The detection limit can reach 1 μg/kg. This method is reliable and could be used as a quality control method for glucosinolates.