蓝莓多酚提取物的抑菌抗炎活性研究

Anti-Proteus mirabilis Effect of Polyphenolic-enriched Blue Berry Extract by In Vitro Evaluation

以分离纯化自牛鼻间隔软骨的多重耐药奇异变形杆菌为主要测试菌种，通过体外抑菌圈实验、最小抑菌浓度实验以及软骨体外培养实验，对蓝莓多酚提取物的抑菌及抗炎活性进行了考察，结果发现蓝莓多酚提取物能够有效抑制奇异变形杆菌、大肠杆菌、枯草芽孢杆菌等革兰氏阳性菌及阴性菌的生长，并能显著降低软骨细胞受奇异变形杆菌感染产生的炎症反应强度。明胶酶谱检验表明蓝莓多酚提取物的抗炎作用主要是由于其能够强烈抑制培养液中软骨细胞问胶原组织蛋白的降解，从而抑制糖胺聚糖（GAG）的释放。

Tbe blue berry（ Vaccinium corymbosum）fruit contains bioactive polyphenols with reported anfi-bactcrlal prop- ertles. This study sought to investigate the anti-bacterial, cartilage-protecting and anti-inflammatory effects of a polyphenolic-enriched blue berry extract using a plate inhibition test, a minimum inhibitory concentration （MIC）test, and an in vitro bovine nasal explant cell culture model. Results showed that not only gram-positive Baeillua subtilis and gram-negative Escherichia coli were inhibited but a multiply antibiotic resistant bacterium Proteus mirabilis was also susceptive to blue berry extract. Gelatin zymography test showed the protease activity was significantly decreased in bovine nasal explant cell culture samples after blue berry extract addition, which was infected with antibiotic resistant bacterium Proteus mirabilis. This confirmed the release inhibition of glycosaminoglycan（ GAG ）, which was a signal of inflammatory, was in according with protease inhibition by blue berry extract.