青霉属真菌P-9生物转化罗汉果总苷的研究

Dtermination of Transformation Products in Microbial Transformation of Cucubitane Triterpene Glycosides from Momordica grosvenori by Penicillium sp P-9 by HPLC

采用青霉属真菌P-9对罗汉果总苷进行生物转化，主要转化产物A、B、C经熔点、比旋度、质谱（MS）、红外光谱（IR）和核磁共振（NMR）波谱分析，鉴定为：A为罗汉果醇-24-O-β-D-葡萄吡喃糖基-（1→2）β-D-葡萄吡喃糖苷；B为罗汉果醇-3-O-β-葡萄吡喃糖基-24O-β-D-葡萄吡喃糖基-（1→2）1B-D-葡萄吡喃糖苷；C为11-酮-罗汉果醇-3-O-β-D-葡萄吡喃糖基-24—O-β-D．葡萄吡喃糖基-（1→2）18．D-葡萄吡喃糖苷，A、B、C三种化合物均未见文献报道。采用高效液相色谱法，以CIS-ODS为色谱柱，乙腈（A）-水（B）作为流动相进行梯度洗脱，检测波长为：203nm，柱温：30℃，流速：1mL／min，进样量：10灿，测得在底物加量为0．5％，28℃，220r／min下摇瓶培养120h，主要产物A、B、C的含量最高分别为1．006、0．293、0．025mg／mL。在选定的色谱条件下转化产物A、B线性范围良好，转化产物A、B的平均加样回收率分别为99．3％．97．8％，RSD分别为1．9％、1．2％。

We found a penicillium sp J-9 which can transform Momordica grosvenori into products A, B and C. Three products were identified by melting point,MS,IR,NMR. The method of HPLC for the transformation products A 、B and C. ZW＆A C18 （250 × 4.60 mm ,5μm）column was used as the stationry phase. The mobile phase was acotonitrile： water, the UV detection wavelength was 203 nm,the column temperature was 30 ℃ ,the flow rate was 1.0 mL/min,the injection volume was 10 μL. The transformation last 120 h under 28 ℃ ,220r/min and substrate at a concertration of 0.5%. The three kinds of products had changeable contents in different time. Results show that the content of products A and B indicates a good linearity under these conditions with average recoveries of （99.3 ± 1.12 ） % and （ 97.8 ± 1.68 ） % and RSDs of 1.9% and 1.2%.