大豆HB基因GmSbh1的分离与生物信息学分析

Isolation and Bioinformatics Analysis of Gm Sbh1 in Soybean

从大豆叶片中分离到Gm Sbh1基因c DNA的完整开放阅读框（ORF）序列,对其编码的氨基酸序列、蛋白质一级、二级结构及互作蛋白等进行了生物信息学分析。结果表明：在Gm Sbh1基因c DNA序列的第156~1 293 bp处有一个完整的ORF,编码379个氨基酸;Gm Sbh1具有同源异型盒（homeoboxbox,HB）基因家族典型的KNOX1、KNOX2、ELK和Homeodomain（HD）结构域;检索到18条与Gm Sbh1核酸序列一致性大于80%的序列;Gm SBH1为亲水性蛋白,不具有信号肽,分子量为42.37 k Da,其中丝氨酸（Ser）、天冬氨酸（Asn）和亮氨酸（Leu）分别为占10%、9.8%和8.4%,可能发生磷酸化的位点分别在Ser（13个）、酪氨酸（Tyr）（2个）和苏氨酸（Thr）（1个）上;二级结构预测结果显示,Gm SBH1序列存在α-螺旋（占39.31%）、β-转角（占5.28%）、延伸链（占8.44%）和无规则卷曲（占46.97%）,不存在跨膜螺旋;蛋白互作预测表明,Gm SBH1与拟南芥中的RPL、BEL1、AT4G32980.1-P、BLH2和BLH3等蛋白的互作程度较高,其中与BEL1共表达的几率较高。

The cDNA sequences of GmSbhl gene in soybean were isolated, and its bioinformatics character was analyzed. The results showed that there was an intact ORF in 156-1 293 bp , and it encoded a 42. 37 kDa protein with 379 amino acids. The GmSBH1 protein contains the KNOX1, KNOX2, ELK and Homeodomain （HD）. There were 18 homologous sequences that have more than 80% identity with GmSbhl. GmSBH1 was a hydrophilic protein without signal peptide and contained 10% Set, 9. 8% Asn and 8.4% I.eu, the phosphorylation sites were located at Set（ 13）, Tyr（2） and Thr（ 1 ）, respectively. Sec- ondary structure prediction results showed that the GmSBttl protein contains alpha-helix （39. 31% ）, beta turn （5.28%）, extended strand（8.44% ） and random coil （46.97%） , but without transmembrane helical structure. The probability of Gm- SH1 interacted with RPL, BELl, AT4G32980. l-P, BLH2 and BLH3 was higher, and coexpression with BELl in Arabibidop-sis.