摘要
fol E基因是高叶酸调控基因,来源于细菌,已证明在西红柿等植物中,提高了叶酸含量。本试验采用农杆菌介导的大豆子叶节法,将fol E基因在大豆受体材料Williams82中表达。共侵染了200个外植体,得到34株转化苗。通过对转化苗进行除草剂(135 mg·L-1)涂抹鉴定,获得14株除草剂抗性转化苗。PCR检测结果显示其中12株为阳性。Southern杂交结果表明,fol E全长c DNA已成功插入到大豆基因组中,并得到2个单拷贝株系。
The folE is a key gene of folic acid pathway, which is derived from bacteria. The function of this gene in the im- proved folate content has been demonstrated in many plants, such as tomato. In this study, we expressed thefblE by employing the soybean eotyledonafy nodes method. We co-cuhivated about 200 explants, and got 34 transgenie plants. Stock of Basra （ 135 mg＇L-1 ） was painted on half the upper surface of tested soybean leaves, and got 14 transgenie plants. PCR analysis showed that 12 of 14 transgenic plants were positive. Southern blot analysis of PCR-positive plants indicated the integration of the folE gene into the soybean genome, and we also got two transgenic lines with only one single copy.
出处
《大豆科学》
CSCD
北大核心
2015年第2期224-227,共4页
Soybean Science
基金
国家高技术研究发展计划“863计划”(2012AA101106-3)
吉林省2012年度博士后科研项目(20120010407)
转基因生物新品种培育重大专项(2013ZX08004)
